| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM60 |
| Uniprot No | Q495X7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-471 aa |
| 活性数据 | MEFVTALVNL QEESSCPICL EYLKDPVTIN CGHNFCRSCL SVSWKDLDDT FPCPVCRFCF PYKSFRRNPQ LRNLTEIAKQ LQIRRSKRKR QKENAMCEKH NQFLTLFCVK DLEILCTQCS FSTKHQKHYI CPIKKAASYH REILEGSLEP LRNNIERVEK VIILQGSKSV ELKKKVEYKR EEINSEFEQI RLFLQNEQEM ILRQIQDEEM NILAKLNENL VELSDYVSTL KHLLREVEGK SVQSNLELLT QAKSMHHKYQ NLKCPELFSF RLTKYGFSLP PQYSGLDRII KPFQVDVILD LNTAHPQLLV SEDRKAVRYE RKKRNICYDP RRFYVCPAVL GSQRFSSGRH YWEVEVGNKP KWILGVCQDC LLRNWQDQPS VLGGFWAIGR YMKSGYVASG PKTTQLLPVV KPSKIGIFLD YELGDLSFYN MNDRSILYTF NDCFTEAVWP YFYTGTDSEP LKICSVSDSE R |
| 分子量 | 55.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM60蛋白的模拟参考文献示例(注：部分信息为示例性概括，建议根据实际文献调整)：
1. **《TRIM60 Modulates Antiviral Innate Immunity by Targeting MAVS for K63-Linked Ubiquitination》**
- 作者：Zhang, Y. et al. (2020)
- 摘要：该研究揭示了TRIM60通过K63位泛素化修饰MAVS蛋白，增强RIG-I样受体(RLR)信号通路介导的I型干扰素产生，从而促进宿主对RNA病毒的先天免疫应答。
2. **《TRIM60 Negatively Regulates NLRP3 Inflammasome Activation in Bacterial Infection》**
- 作者：Li, X. et al. (2021)
- 摘要：研究表明，TRIM60通过靶向NLRP3炎症小体，抑制其过度活化并减少促炎因子IL-1β的释放，在细菌感染中发挥免疫稳态调控作用。
3. **《TRIM60 Promotes Tumorigenesis via p53 Ubiquitination and Degradation》**
- 作者：Wang, Q. et al. (2019)
- 摘要：发现TRIM60作为E3泛素连接酶促进p53蛋白的泛素化降解，导致细胞周期调控异常，与非小细胞肺癌的发生发展密切相关。
4. **《Structural Insights into the TRIM60 Coiled-Coil Domain Mediating Self-Assembly》**
- 作者：Chen, L. et al. (2022)
- 摘要：解析了TRIM60卷曲螺旋域的三维结构，阐明其通过自组装形成多聚体以行使功能，为开发靶向TRIM60的分子工具奠定基础。
*提示*：以上内容基于研究领域常见方向模拟，具体文献需通过PubMed、Google Scholar等平台以“TRIM60”为关键词检索。
Trim60. a member of the tripartite motif (TRIM) protein family, is characterized by its conserved N-terminal RING finger, B-box, and coiled-coil domains. These structural components enable TRIM60 to function as an E3 ubiquitin ligase, mediating post-translational protein modifications through ubiquitination. While the biological roles of TRIM60 remain less explored compared to other TRIM proteins, emerging evidence suggests its involvement in innate immunity, antiviral defense, and cellular homeostasis. Studies indicate that TRIM60 may regulate inflammatory pathways by interacting with key signaling molecules like MAVS or STING, potentially modulating interferon production. Its expression appears tissue-specific, with higher levels reported in immune-related organs and testes, hinting at roles in both immune responses and reproductive biology. Some research links Trim60 to autophagy, where it may target proteins for degradation via ubiquitin-dependent mechanisms. Notably, murine models show that Trim60 deficiency alters susceptibility to viral infections and inflammatory conditions, though precise mechanisms require further validation. Phylogenetically, Trim60 demonstrates conservation across vertebrates, emphasizing its evolutionary significance. Current knowledge gaps include its substrate specificity, regulatory networks, and relevance to human diseases. Ongoing studies aim to clarify its interplay with pathogens, potential tumor-suppressive or oncogenic roles, and contribution to autoimmune disorders.
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