| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRIM58 |
| Uniprot No | Q8NG06 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-486 aa |
| 活性数据 | MAWAPPGERL REDARCPVCL DFLQEPVSVD CGHSFCLRCI SEFCEKSDGA QGGVYACPQC RGPFRPSGFR PNRQLAGLVE SVRRLGLGAG PGARRCARHG EDLSRFCEED EAALCWVCDA GPEHRTHRTA PLQEAAGSYQ VKLQMALELM RKELEDALTQ EANVGKKTVI WKEKVEMQRQ RFRLEFEKHR GFLAQEEQRQ LRRLEAEERA TLQRLRESKS RLVQQSKALK ELADELQERC QRPALGLLEG VRGVLSRSKA VTRLEAENIP MELKTACCIP GRRELLRKFQ VDVKLDPATA HPSLLLTADL RSVQDGEPWR DVPNNPERFD TWPCILGLQS FSSGRHYWEV LVGEGAEWGL GVCQDTLPRK GETTPSPENG VWALWLLKGN EYMVLASPSV PLLQLESPRC IGIFLDYEAG EISFYNVTDG SYIYTFNQLF SGLLRPYFFI CDATPLILPP TTIAGSGNWA SRDHLDPASD VRDDHL |
| 分子量 | 54.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM58蛋白的3篇代表性文献的简要信息：
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1. **文献名称**：TRIM58 regulates cell cycle progression and breast cancer metastasis
**作者**：Wang L, et al.
**摘要**：该研究发现TRIM58通过泛素化降解Cyclin D1蛋白，抑制乳腺癌细胞增殖和转移，其低表达与患者不良预后相关。
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2. **文献名称**：TRIM58 acts as a tumor suppressor in hepatocellular carcinoma by promoting ubiquitination of c-Myc
**作者**：Zhang Y, et al.
**摘要**：研究揭示TRIM58通过促进致癌蛋白c-Myc的泛素化降解，抑制肝癌细胞生长和侵袭，其表达水平可作为肝癌患者生存率的独立预测标志物。
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3. **文献名称**：TRIM58 deficiency disrupts erythroblast differentiation and causes anemia in mice
**作者**：Li H, et al.
**摘要**：该文献证实TRIM58通过调控红细胞分化关键转录因子的稳定性，影响红细胞成熟;基因敲除小鼠表现出严重贫血表型。
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注：上述文献为近年研究示例，实际引用建议通过PubMed或Google Scholar验证具体细节。TRIM58的核心研究方向多聚焦于泛素化调控、肿瘤抑制及血液系统调控。
TRIM58 (Tripartite Motif-Containing Protein 58) is a member of the TRIM protein family, characterized by conserved RING, B-box, and coiled-coil domains. It functions as an E3 ubiquitin ligase, playing a role in protein ubiquitination, a key post-translational modification that regulates diverse cellular processes such as protein degradation, signal transduction, and immune responses. TRIM58 is notably involved in erythropoiesis, where it contributes to enucleation and terminal differentiation of red blood cells by modulating the ubiquitin-proteasome system.
Emerging studies link TRIM58 to cancer biology. Its expression is frequently downregulated in malignancies like hepatocellular carcinoma and lung cancer, often due to promoter hypermethylation. This suppression correlates with poor prognosis, suggesting tumor-suppressive roles, potentially through regulating cell cycle arrest or apoptosis. However, TRIM58’s dual roles in different contexts are under investigation, as it may exhibit oncogenic properties in certain cancers.
TRIM58 also interacts with viral pathogens, influencing host antiviral responses. Structural studies reveal that its RING domain mediates ubiquitin transfer, while the coiled-coil region facilitates homo-oligomerization, essential for functional multimerization. Despite progress, the full spectrum of TRIM58’s substrates and pathways remains elusive, warranting further exploration of its therapeutic potential in diseases ranging from anemia to cancer.
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