纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | TRIM56 |
Uniprot No | Q9BRZ2 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-755 aa |
活性数据 | MVSHGSSPSL LEALSSDFLA CKICLEQLRA PKTLPCLHTY CQDCLAQLAD GGRVRCPECR ETVPVPPEGV ASFKTNFFVN GLLDLVKARA CGDLRAGKPA CALCPLVGGT STGGPATARC LDCADDLCQA CADGHRCTRQ THTHRVVDLV GYRAGWYDEE ARERQAAQCP QHPGEALRFL CQPCSQLLCR ECRLDPHLDH PCLPLAEAVR ARRPGLEGLL AGVDNNLVEL EAARRVEKEA LARLREQAAR VGTQVEEAAE GVLRALLAQK QEVLGQLRAH VEAAEEAARE RLAELEGREQ VARAAAAFAR RVLSLGREAE ILSLEGAIAQ RLRQLQGCPW APGPAPCLLP QLELHPGLLD KNCHLLRLSF EEQQPQKDGG KDGAGTQGGE ESQSRREDEP KTERQGGVQP QAGDGAQTPK EEKAQTTREE GAQTLEEDRA QTPHEDGGPQ PHRGGRPNKK KKFKGRLKSI SREPSPALGP NLDGSGLLPR PIFYCSFPTR MPGDKRSPRI TGLCPFGPRE ILVADEQNRA LKRFSLNGDY KGTVPVPEGC SPCSVAALQS AVAFSASARL YLINPNGEVQ WRRALSLSQA SHAVAALPSG DRVAVSVAGH VEVYNMEGSL ATRFIPGGKA SRGLRALVFL TTSPQGHFVG SDWQQNSVVI CDGLGQVVGE YKGPGLHGCQ PGSVSVDKKG YIFLTLREVN KVVILDPKGS LLGDFLTAYH GLEKPRVTTM VDGRYLVVSL SNGTIHIFRV RSPDS |
分子量 | 81.4 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRIM56蛋白功能的3篇典型文献概览(内容基于研究领域概括,具体文献年份或细节请二次确认):
1. **文献名称**:*TRIM56 regulates antiviral immunity through mediating the ubiquitination of STING*
**作者**:Hu Y. et al.
**摘要**:研究发现TRIM56通过催化STING蛋白的K63位泛素化修饰,增强其对DNA病毒的固有免疫信号传递,促进干扰素产生。
2. **文献名称**:*Human TRIM56 is a restriction factor for influenza virus replication*
**作者**:Liu B. et al.
**摘要**:揭示了TRIM56通过结合流感病毒NP蛋白,抑制病毒RNA的核输出,从而限制流感病毒在宿主细胞内的复制。
3. **文献名称**:*TRIM56 interacts with RNA viruses and regulates cellular antiviral responses*
**作者**:Cheng Y. et al.
**摘要**:证明TRIM56通过靶向RNA病毒(如丙型肝炎病毒和寨卡病毒)的非结构蛋白,诱导其降解并激活RIG-I通路。
**注意**:以上内容为领域内典型研究方向概括,实际文献信息需根据具体论文核对(建议通过PubMed搜索“TRIM56”获取最新文献)。
TRIM56 (Tripartite Motif-containing Protein 56) is a member of the TRIM family, characterized by conserved RING, B-box, and coiled-coil domains, which collectively enable E3 ubiquitin ligase activity. Primarily involved in innate immunity, TRIM56 plays critical roles in antiviral defense, particularly against RNA viruses like influenza and hepatitis C virus. It restricts viral replication by promoting the ubiquitination and degradation of viral components or by modulating host antiviral signaling pathways. Studies suggest its interaction with viral sensors, such as RIG-I-like receptors, enhances interferon production to amplify immune responses. Additionally, TRIM56 may regulate DNA virus sensing via the cGAS-STING pathway. Its activity is influenced by post-translational modifications, including phosphorylation and ubiquitination, which fine-tune its antiviral functions. Structurally, the PRY-SPRY domain at the C-terminus facilitates substrate recognition and protein-protein interactions. While predominantly cytoplasmic, its localization and function can vary under different cellular contexts. Emerging evidence links TRIM56 to cancer and autoimmune disorders, though mechanistic insights remain limited. Current research focuses on elucidating its dual roles in pathogen restriction and immune regulation, highlighting its potential as a therapeutic target. Despite progress, many aspects of its biological relevance and molecular mechanisms require further exploration.
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