| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHX35 |
| Uniprot No | Q9H5Z1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-703aa |
| 氨基酸序列 | MAAPVGPVKFWRPGTEGPGVSISEERQSLAENSGTTVVYNPYAALSIEQQRQKLPVFKLRNHILYLIENYQTVVIVGETGCGKSTQIPQYLAEAGWTAEGRVVGVTQPRRVAAVTVAGRVAEERGAVLGHEVGYCIRFDDCTDQLATRIKFLTDGMLVREMMVDPLLTKYSVIMLDEAHERTLYTDIAIGLLKKIQKKRGDLRLIVASATLDADKFRDFFNQNETSDPARDTCVILTVEGRTFPVDIFYLQSPVPDYIKSTVETVVKIHQTEGDGDVLAFLTGQEEVETVVSMLIEQARALARTGMKRHLRVLPMYAGLPSFEQMKVFERVSRSVRKVIVATNVAETSITISGIVYVIDCGFVKLRAYNPRTAIECLVVVPVSQASANQRAGRGGRSRSGKCYRLYTEEAFDKLPQSTVPEMQRSNLAPVILQLKALGIDNVLRFHFMSPPPAQSMVQALELLYALGGLDKDCRLTEPLGMRIAEFPLNPMFAKMLLESGNFGCSQEILSIAAMMQIQNIFVVPPNQKSHAIRVHRKFAVEEGDHLTMLNIYEAFIKHNKDSKWCQEHFLNYKGLVRAATVREQLKKLLVKFQVPRKSSEGDPDLVLRCIVSGFFANAARFHSTGAYRTIRDDHELHIHPASVLYAEKPPRWVIYNEVIQTSKYYMRDVTAIESAWLLELAPHFYQQGTHLSLKAKRAKVQDP |
| 分子量 | 103.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于DHX35蛋白相关研究的常见方向构建的模拟参考文献，仅供参考。实际文献需通过学术数据库(如PubMed、Google Scholar)验证：
1. **文献名称**：*Structural Insights into Human DHX35 Helicase in RNA Processing*
**作者**：Smith A, et al.
**摘要**：解析了重组人DHX35蛋白的晶体结构，揭示其通过DEAH结构域结合RNA，并参与pre-mRNA剪接的分子机制。
2. **文献名称**：*DHX35 Activates Antiviral Immunity by Recognizing Viral RNA*
**作者**：Zhang L, et al.
**摘要**：证明DHX35通过识别RNA病毒的双链RNA结构，触发MAVS信号通路，促进Ⅰ型干扰素产生，发挥抗病毒作用。
3. **文献名称**：*Oncogenic Role of DHX35 in Colorectal Cancer Proliferation*
**作者**：Wang X, et al.
**摘要**：发现DHX35在结直肠癌中高表达，通过调控Wnt/β-catenin信号通路促进肿瘤细胞增殖和转移。
4. **文献名称**：*Recombinant DHX35 Purification and Enzymatic Characterization*
**作者**：Lee J, et al.
**摘要**：优化了重组DHX35蛋白在大肠杆菌中的表达与纯化工艺，并验证其ATP依赖的RNA解旋酶活性。
**注意**：以上文献为模拟内容，实际研究请查阅权威数据库。建议使用关键词**"DHX35 helicase"、"recombinant DHX35"**检索真实文献。
**Recombinant human DHX35 protein** is a genetically engineered version of the endogenous DEAH-box helicase 35. a member of the DEAH-box RNA helicase family. DHX35 plays a critical role in RNA metabolism, including pre-mRNA splicing, RNA processing, and ribosome biogenesis. It utilizes ATP hydrolysis to unwind RNA secondary structures or dissociate RNA-protein complexes, facilitating RNA remodeling during gene expression. Studies suggest DHX35 interacts with spliceosomal components, potentially regulating alternative splicing events linked to cellular differentiation, proliferation, and stress responses. Dysregulation of DHX35 has been associated with cancers and neurological disorders, highlighting its functional significance.
The recombinant protein is typically expressed in bacterial or eukaryotic systems, enabling in vitro studies on its biochemical properties, RNA-binding specificity, and helicase activity. Its conserved helicase core domain, comprising RecA-like domains and a winged-helix motif, drives ATP-dependent RNA unwinding. Researchers use recombinant DHX35 to investigate its role in splicing fidelity, ribosome assembly, or viral RNA sensing, as well as to screen for inhibitors in therapeutic contexts. Its versatility as a tool enhances understanding of RNA helicase mechanisms and disease-related RNA dysregulation.
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