| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHRSX |
| Uniprot No | Q8N5I4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-330aa |
| 氨基酸序列 | MSPLSAARAALRVYAVGAAVILAQLLRRCRGGFLEPVFPPRPDRVAIVTGGTDGIGYSTAKHLARLGMHVIIAGNNDSKAKQVVSKIKEETLNDKVEFLYCDLASMTSIRQFVQKFKMKKIPLHVLINNAGVMMVPQRKTRDGFEEHFGLNYLGHFLLTNLLLDTLKESGSPGHSARVVTVSSATHYVAELNMDDLQSSACYSPHAAYAQSKLALVLFTYHLQRLLAAEGSHVTANVVDPGVVNTDLYKHVFWATRLAKKLLGWLLFKTPDEGAWTSIYAAVTPELEGVGGRYLYNEKETKSLHVTYNQKLQQQLWSKSCEMTGVLDVTL |
| 分子量 | 62.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DHRSX蛋白的假设性参考文献示例，建议通过学术数据库(如PubMed、Google Scholar)核实具体研究：
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1. **文献名称**：*Characterization of recombinant human DHRSX: a short-chain dehydrogenase/reductase with retinol dehydrogenase activity*
**作者**：Ruiz A. et al.
**摘要**：研究了重组人DHRSX蛋白在HEK293细胞中的表达，发现其具有视黄醇脱氢酶活性，可将视黄醇转化为视黄醛，提示其在维甲酸代谢中的潜在作用。
2. **文献名称**：*Cloning, expression, and purification of human DHRSX protein in Escherichia coli*
**作者**：Kim J. et al.
**摘要**：报道了在大肠杆菌中成功表达和纯化可溶性重组人DHRSX蛋白，通过条件优化获得高活性蛋白，为后续功能研究提供基础。
3. **文献名称**：*DHRSX suppresses hepatocellular carcinoma progression through modulating lipid metabolism*
**作者**：Wang L. et al.
**摘要**：通过体外实验验证重组人DHRSX蛋白的功能，发现其通过调节脂质代谢抑制肝癌细胞的增殖与迁移，具有潜在抑癌作用。
4. **文献名称**：*Structural insights into the catalytic mechanism of human DHRSX*
**作者**：Patel M. et al.
**摘要**：解析了重组DHRSX蛋白的晶体结构，阐明其与辅因子NADP+的结合模式及底物识别机制，为酶学功能研究提供结构基础。
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**注意**：以上文献为示例性质，实际研究请参考权威数据库。若需具体文献信息，建议使用关键词“recombinant human DHRSX”在PubMed或Web of Science中检索。
Recombinant human DHRSX protein is a genetically engineered form of the dehydrogenase/reductase (SDR) family member X, encoded by the *DHRSX* gene located on the pseudoautosomal region (PAR1) of X and Y chromosomes. Initially identified as a testis-expressed protein, DHRSX shares homology with microbial enzymes involved in retinoid and steroid metabolism. It contains conserved motifs typical of NAD(P)H-dependent oxidoreductases, including a Rossmann fold for cofactor binding, and exists as a homodimer in its active state. While its precise physiological role remains unclear, DHRSX is hypothesized to regulate cellular differentiation, lipid metabolism, or signaling pathways (e.g., retinoic acid signaling), potentially influencing embryonic development and tissue homeostasis. Recombinant versions are produced in bacterial or mammalian expression systems to study its enzymatic activity, substrate specificity, and interactions. Interest in DHRSX stems from its links to X-Y chromosome evolution and clinical implications: mutations are associated with sex development disorders, neurodevelopmental conditions, and cancers. Current research focuses on elucidating its biochemical mechanisms and therapeutic potential. However, functional studies remain limited, partly due to its complex genomic context and low natural expression levels in most tissues.
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