| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHRS4L2 |
| Uniprot No | Q6PKH6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-232aa |
| 氨基酸序列 | ASSRMTRRDPLTNKVALVTASTDGIGFAIARRLAQDRAHVVVSSRKQQNVDQAVATLQGEGLSVTGTVCHVGKAEDRERLVAMAVKLHGGIDILVSNAAVNPFFGSLMDVTEEVWDKTLDINVKAPALMTKAVVPEMEKRGGGSVVIVSSIAAFSPSPGFSPYNVSKTALLGLNNTLAIELAPRNIRVNCLHLDLSRLASAGCSGWTRKKRKA |
| 分子量 | 23.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DHRS4L2蛋白的3篇文献摘要，基于知识库内容模拟生成：
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1. **文献名称**：*"Expression and Purification of Recombinant Human DHRS4L2 in Escherichia coli for Functional Analysis"*
**作者**：Li, X., Zhang, Y., Wang, H.
**摘要**：本研究通过克隆DHRS4L2基因至大肠杆菌表达系统，成功表达并纯化了可溶性的重组人DHRS4L2蛋白。通过酶活实验发现该蛋白对全反式视黄醛(atRAL)具有特异性还原酶活性，推测其参与视网膜色素上皮细胞的视黄醇代谢保护机制。
2. **文献名称**：*"Structural Characterization of DHRS4L2 and Its Role in Regulating Cellular Retinoic Acid Homeostasis"*
**作者**：Chen, J., Liu, R., Wei, P.
**摘要**：作者解析了重组DHRS4L2蛋白的晶体结构，揭示了其与辅酶NADPH结合的活性位点。功能研究表明，DHRS4L2通过降解过量视黄酸(RA)维持细胞内RA平衡，敲低该蛋白导致RA信号通路异常激活，提示其在细胞分化中的潜在调控作用。
3. **文献名称**：*"DHRS4L2 Acts as a Tumor Suppressor in Hepatocellular Carcinoma by Modulating Reactive Oxygen Species"*
**作者**：Guo, S., Yang, L., Zhou, M.
**摘要**：研究发现肝癌组织中DHRS4L2表达显著下调。体外实验表明，重组DHRS4L2蛋白的过表达可降低细胞内ROS水平并抑制肝癌细胞增殖和迁移，提示其通过抗氧化途径发挥抑癌功能，可能成为肝癌治疗的潜在靶点。
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注：以上文献为模拟生成，实际研究请参考具体已发表论文数据库(如PubMed)。如需真实文献，建议在专业平台检索关键词“DHRS4L2 recombinant protein”或“DHRS4L2 function”。
Human dehydrogenase/reductase (SDR family) member 4-like 2 (DHRS4L2) is a poorly characterized protein belonging to the short-chain dehydrogenase/reductase (SDR) superfamily. Its gene is located in a genomic cluster near DHRS4 on chromosome 14. likely arising from gene duplication events. Though structurally homologous to DHRS4. DHRS4L2 exhibits distinct expression patterns and functional properties. Preliminary studies suggest involvement in retinoid metabolism, potentially modulating retinoic acid synthesis or degradation, which links it to cellular differentiation and proliferation pathways. As an NADPH-dependent oxidoreductase, it may also participate in redox homeostasis and steroid hormone metabolism.
Recombinant DHRS4L2 protein is typically produced in E. coli or mammalian expression systems for functional studies. Researchers employ it to investigate substrate specificity, enzymatic kinetics, and structural features through techniques like X-ray crystallography. Its interactions with cellular retinol-binding proteins and potential role in lipid metabolism have drawn interest in metabolic disorders and cancer research. Interestingly, DHRS4L2 expression appears epigenetically regulated in certain cancers, hinting at diagnostic or therapeutic relevance. However, its precise physiological roles remain unclear, prompting ongoing research to clarify its biological significance compared to related SDR enzymes. Current applications include biochemical characterization, antibody development, and pathway analysis in disease models.
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