| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHX16 |
| Uniprot No | O60231 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1041aa |
| 氨基酸序列 | MATPAGLERWVQDELHSVLGLSERHVAQFLIGTAQRCTSAEEFVQRLRDTDTLDLSGPARDFALRLWNKVPRKAVVEKPARAAEREARALLEKNRSYRLLEDSEESSEETVSRAGSSLQKKRKKRKHLRKKREEEEEEEASEKGKKKTGGSKQQTEKPESEDEWERTERERLQDLEERDAFAERVRQRDKDRTRNVLERSDKKAYEEAQKRLKMAEEDRKAMVPELRKKSRREYLAKREREKLEDLEAELADEEFLFGDVELSRHERQELKYKRRVRDLAREYRAAGEQEKLEATNRYHMPKETRGQPARAVDLVEEESGAPGEEQRRWEEARLGAASLKFGARDAASQEPKYQLVLEEEETIEFVRATQLQGDEEPSAPPTSTQAQQKESIQAVRRSLPVFPFREELLAAIANHQVLIIEGETGSGKTTQIPQYLFEEGYTNKGMKIACTQPRRVAAMSVAARVAREMGVKLGNEVGYSIRFEDCTSERTVLRYMTDGMLLREFLSEPDLASYSVVMVDEAHERTLHTDILFGLIKDVARFRPELKVLVASATMDTARFSTFFDDAPVFRIPGRRFPVDIFYTKAPEADYLEACVVSVLQIHVTQPPGDILVFLTGQEEIEAACEMLQDRCRRLGSKIRELLVLPIYANLPSDMQARIFQPTPPGARKVVVATNIAETSLTIEGIIYVLDPGFCKQKSYNPRTGMESLTVTPCSKASANQRAGRAGRVAAGKCFRLYTAWAYQHELEETTVPEIQRTSLGNVVLLLKSLGIHDLMHFDFLDPPPYETLLLALEQLYALGALNHLGELTTSGRKMAELPVDPMLSKMILASEKYSCSEEILTVAAMLSVNNSIFYRPKDKVVHADNARVNFFLPGGDHLVLLNVYTQWAESGYSSQWCYENFVQFRSMRRARDVREQLEGLLERVEVGLSSCQGDYIRVRKAITAGYFYHTARLTRSGYRTVKQQQTVFIHPNSSLFEQQPRWLLYHELVLTTKEFMRQVLEIESSWLLEVAPHYYKAKELEDPHAKKMPKKIGKTREELG |
| 分子量 | 119.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人DHX16蛋白的研究文献概览(基于公开学术信息虚拟示例，建议核实真实文献)：
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**1. 文献名称**：*DHX16 Is Required for Pre-mRNA Splicing and Maintenance of Retinal Homeostasis*
**作者**：G. Hautbergue, R.D. Barrass, J.D. Beggs (2006)
**摘要**：研究证实重组人DHX16作为剪接体的核心RNA解旋酶，通过体外重组蛋白实验揭示其通过水解ATP促进pre-mRNA剪接的构象变化，为DHX16在RNA代谢中的核心作用提供证据。
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**2. 文献名称**：*Structural Insights into the Mechanism of DHX16 Helicase in Spliceosome Assembly*
**作者**：Y. Chen, L. Zhang (2020)
**摘要**：通过冷冻电镜解析重组人DHX16蛋白的3D结构，发现其DEAH结构域与RNA结合的动态模式，阐明了DHX16在剪接体组装中协调RNA解旋和蛋白质相互作用的分子机制。
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**3. 文献名称**：*DHX16 Recognizes Viral RNA and Trigres Antiviral Immune Response*
**作者**：M. Zhang, H. Jiang (2015)
**摘要**：研究表明重组人DHX16蛋白可直接结合病毒RNA，激活MAVS信号通路从而诱导I型干扰素产生，首次揭示DHX16在宿主先天免疫中的双重功能(RNA解旋与免疫调控)。
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**提示**：上述示例为模拟生成，实际文献需通过PubMed、Web of Science等平台检索关键词(如“DHX16 recombinant human”、“DHX16 helicase function”)获取真实数据。
Human DHX16. a member of the DEAH-box RNA helicase family, plays critical roles in RNA metabolism, including pre-mRNA splicing, RNA processing, and degradation. It is an ATP-dependent helicase involved in spliceosome dynamics, particularly during the catalytic activation and disassembly of the spliceosome. DHX16 interacts with spliceosomal components (e.g., U2 snRNP proteins) and participates in proofreading or remodeling RNA-RNA or RNA-protein interactions to ensure splicing fidelity. Dysregulation of DHX16 has been linked to diseases such as cancer and neurodegenerative disorders, where aberrant splicing contributes to pathogenesis. Recombinant human DHX16 protein, typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., insect cells), retains enzymatic activity and is widely used to study RNA splicing mechanisms in vitro. Its applications include elucidating helicase functions, identifying binding partners via pull-down assays, and screening for modulators in drug discovery. Structural studies using recombinant DHX16 have also advanced understanding of its conserved DEAH helicase domain and regulatory C-terminal region. Further research on recombinant DHX16 may uncover therapeutic strategies targeting splicing-related diseases.
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