| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHRS7B |
| Uniprot No | Q6IAN0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-325aa |
| 氨基酸序列 | MVSPATRKSLPKVKAMDFITSTAILPLLFGCLGVFGLFRLLQWVRGKAYLRNAVVVITGATSGLGKECAKVFYAAGAKLVLCGRNGGALEELIRELTASHATKVQTHKPYLVTFDLTDSGAIVAAAAEILQCFGYVDILVNNAGISYRGTIMDTTVDVDKRVMETNYFGPVALTKALLPSMIKRRQGHIVAISSIQGKMSIPFRSAYAASKHATQAFFDCLRAEMEQYEIEVTVISPGYIHTNLSVNAITADGSRYGVMDTTTAQGRSPVEVAQDVLAAVGKKKKDVILADLLPSLAVYLRTLAPGLFFSLMASRARKERKSKNS |
| 分子量 | 61.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DHRS7B蛋白的3篇参考文献的简要概括：
1. **文献名称**: "Cloning, Expression, and Characterization of Human DHRS7B: A Novel Short-Chain Dehydrogenase/Reductase with Retinal Reductase Activity"
**作者**: Zhao et al.
**摘要**: 该研究成功克隆并表达了重组人DHRS7B蛋白，发现其在体外可将视黄醇转化为视黄酸，揭示了其在类维生素A代谢中的潜在作用。
2. **文献名称**: "Structural and Functional Analysis of DHRS7B Reveals a Role in Lipid Metabolism"
**作者**: Smith et al.
**摘要**: 通过X射线晶体学解析了DHRS7B的三维结构，并证明其通过催化脂质底物的还原反应参与脂代谢调控，可能与代谢性疾病相关。
3. **文献名称**: "Recombinant DHRS7B Production in E. coli: Optimization and Enzymatic Assays"
**作者**: Chen & Wang
**摘要**: 该文献优化了DHRS7B在大肠杆菌中的可溶性表达条件，建立了纯化流程，并验证其对羟基类固醇等底物的催化活性。
注：上述文献为示例，若需实际文献，建议通过PubMed或Google Scholar以关键词“DHRS7B recombinant”、“Human DHRS7B function”检索。
Human dehydrogenase/reductase 7B (DHRS7B) is a member of the short-chain dehydrogenase/reductase (SDR) superfamily, enzymes known for their roles in catalyzing NAD+/NADP+-dependent oxidation-reduction reactions. This protein is encoded by the DHRS7B gene, located on chromosome 17 in humans, and shares structural features with other SDR members, including a conserved Rossmann-fold motif for cofactor binding. While its precise biological function remains under investigation, DHRS7B is hypothesized to participate in metabolic pathways, potentially involving steroid hormones, retinoids, or xenobiotic compounds, given the enzymatic activity observed in homologous proteins.
Recombinant human DHRS7B protein is artificially expressed in heterologous systems (e.g., E. coli or mammalian cells) to enable functional studies. Its production facilitates research into substrate specificity, enzymatic kinetics, and interactions with signaling molecules. Emerging evidence suggests DHRS7B may influence cellular processes like proliferation and differentiation, with potential links to cancer, metabolic disorders, or neurological diseases. However, its physiological substrates and regulatory mechanisms are not fully elucidated, highlighting the need for further exploration.
Current studies focus on characterizing its 3D structure, tissue distribution (notably in liver, kidney, and brain), and interplay with metabolic networks. Recombinant DHRS7B serves as a key tool for unraveling its role in health and disease, offering insights for therapeutic targeting.
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