| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHX15 |
| Uniprot No | O43143 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-795aa |
| 氨基酸序列 | MSKRHRLDLG EDYPSGKKRA GTDGKDRDRD RDREDRSKDR DRERDRGDRE REREKEKEKE LRASTNAMLI SAGLPPLKAS HSAHSTHSAH STHSTHSAHS THAGHAGHTS LPQCINPFTN LPHTPRYYDI LKKRLQLPVW EYKDRFTDIL VRHQSFVLVG ETGSGKTTQI PQWCVEYMRS LPGPKRGVAC TQPRRVAAMS VAQRVADEMD VMLGQEVGYS IRFEDCSSAK TILKYMTDGM LLREAMNDPL LERYGVIILD EAHERTLATD ILMGVLKEVV RQRSDLKVIV MSATLDAGKF QIYFDNCPLL TIPGRTHPVE IFYTPEPERD YLEAAIRTVI QIHMCEEEEG DLLLFLTGQE EIDEACKRIK REVDDLGPEV GDIKIIPLYS TLPPQQQQRI FEPPPPKKQN GAIGRKVVVS TNIAETSLTI DGVVFVIDPG FAKQKVYNPR IRVESLLVTA ISKASAQQRA GRAGRTRPGK CFRLYTEKAY KTEMQDNTYP EILRSNLGSV VLQLKKLGID DLVHFDFMDP PAPETLMRAL ELLNYLAALN DDGDLTELGS MMAEFPLDPQ LAKMVIASCD YNCSNEVLSI TAMLSVPQCF VRPTEAKKAA DEAKMRFAHI DGDHLTLLNV YHAFKQNHES VQWCYDNFIN YRSLMSADNV RQQLSRIMDR FNLPRRSTDF TSRDYYINIR KALVTGYFMQ VAHLERTGHY LTVKDNQVVQ LHPSTVLDHK PEWVLYNEFV LTTKNYIRTC TDIKPEWLVK IAPQYYDMSN FPQCEAKRQL DRIIAKLQSK EYSQY |
| 分子量 | 90.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人DHX15蛋白研究的代表性文献，涵盖其功能、结构及应用方向：
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1. **"Structural basis for RNA surveillance by the human RNA helicase DHX15"**
*作者*: Tetsuo Mashimo, Masahiro Higashi, Hiroshi Kido*
*摘要*: 本研究解析了人源DHX15蛋白的晶体结构，揭示其RNA结合域的构象变化机制，并证明其通过ATP依赖的解旋酶活性参与核糖体RNA加工及错误RNA的降解，为理解RNA质量控制机制提供结构基础。
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2. **"DHX15 senses double-stranded RNA in myeloid dendritic cells and promotes antiviral immunity"**
*作者*: Xiaojuan Wang, Yuhao Zhou, Qin Zhang*
*摘要*: 发现重组人DHX15蛋白在树突状细胞中可作为病毒双链RNA的识别受体，激活MAVS依赖性干扰素信号通路，阐明了其在抗RNA病毒天然免疫中的关键作用。
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3. **"The RNA helicase DHX15 promotes colorectal cancer metastasis by regulating alternative splicing of key oncogenic genes"**
*作者*: Li Chen, Emily L. May, Kevin M. Shokat*
*摘要*: 通过重组DHX15蛋白的功能实验，证实其通过调控结直肠癌相关基因(如MYC、CD44)的可变剪接，驱动癌症转移，提示其作为肿瘤治疗靶点的潜力。
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*注：以上文献信息为示例性总结，实际文献标题及内容需根据具体研究补充。建议通过PubMed/Google Scholar以“recombinant DHX15”“DHX15 function”为关键词检索最新研究。*
DHX15. a member of the DEAH-box helicase family, is an ATP-dependent RNA helicase involved in diverse RNA metabolic processes. It plays critical roles in pre-mRNA splicing, ribosome biogenesis, and innate immune responses. Structurally, it contains conserved helicase domains (DEAH and HelicC) that enable RNA binding, unwinding, and remodeling. In antiviral immunity, DHX15 interacts with viral RNA and activates downstream signaling pathways (e.g., RIG-I/MDA5) to trigger IFN production. Its role extends to maintaining genomic stability and regulating cell proliferation. Dysregulation of DHX15 is implicated in cancers, viral infections, and autoimmune disorders. Recombinant human DHX15 protein is typically produced in *E. coli* or mammalian expression systems, enabling functional studies on its helicase activity, RNA-protein interactions, and immune regulatory mechanisms. This purified protein serves as a vital tool for investigating RNA processing pathways, host-pathogen interactions, and therapeutic target validation. Its biochemical properties are further utilized in high-throughput screening for antiviral or anticancer compounds. The gene encoding DHX15 is located on chromosome 22q13.1. with mutations linked to developmental disorders and hematologic malignancies. Ongoing research focuses on its dual roles in RNA homeostasis and immune signaling.
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