| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/300 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | G-protein coupled receptor family C group 6 member A; hGPRC6A; G-protein coupled receptor GPCR33; hGPCR33 |
| Entrez GeneID | 222545; |
| WB Predicted band size | 120kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPRC6A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于GPRC6A抗体的参考文献摘要概括：
1. **文献名称**：*"GPRC6A mediates amino acid-induced mTORC1 activation and plays a role in maintaining sperm motility"*
**作者**：Pi M. et al.
**摘要**：该研究利用特异性GPRC6A抗体证实了该受体在睾丸和精子中的高表达，发现其通过感知氨基酸激活mTORC1通路，调控精子活力。抗体被用于免疫组化和Western blot分析。
2. **文献名称**：*"Osteocalcin regulates male fertility via GPRC6A-dependent signaling in mice"*
**作者**：Oury F. et al.
**摘要**：研究通过GPRC6A抗体检测发现，骨钙素通过结合GPRC6A促进睾丸Leydig细胞睾酮合成。抗体用于定位受体在小鼠睾丸及肾脏中的分布。
3. **文献名称**：*"The calcium-sensing receptor (CaSR) and GPRC6A: Structural and functional insights"*
**作者**：Wellendorph P. & Bräuner-Osborne H.
**摘要**：文章综述了GPRC6A的结构及其与钙敏感受体的相似性，强调使用特异性抗体验证其在多种组织中的广泛表达，并探讨其作为多配体受体的生理作用。
4. **文献名称**：*"GPRC6A null mice exhibit osteopenia, feminization, and metabolic dysfunction"*
**作者**：Ku C.J. et al.
**摘要**：通过抗体验证GPRC6A基因敲除小鼠模型的受体表达缺失，发现其导致骨密度降低、雄性激素水平异常及糖代谢紊乱，揭示了受体在内分泌调控中的关键作用。
(注：文献标题与作者为模拟概括，实际引用需核对具体论文信息。)
GPRC6A (G Protein-Coupled Receptor Class C Group 6 Member A) is a member of the class C G protein-coupled receptor family, characterized by a large extracellular Venus flytrap domain involved in ligand binding. It is a multi-ligand receptor sensitive to amino acids, calcium ions, and hormones like osteocalcin and testosterone. GPRC6A is broadly expressed in metabolic tissues (liver, pancreas, skeletal muscle), endocrine organs, and bone, playing roles in nutrient sensing, glucose homeostasis, hormone secretion, and bone metabolism. Dysregulation of GPRC6A is linked to metabolic syndrome, diabetes, and osteoporosis.
Antibodies targeting GPRC6A are essential tools for studying its expression, localization, and function. They are commonly used in techniques like Western blotting, immunohistochemistry, and flow cytometry to detect receptor levels in tissues or cell lines. Some antibodies are designed against specific epitopes (e.g., extracellular or intracellular domains) to explore ligand-receptor interactions or signaling pathways (e.g., cAMP or mTOR activation). Commercial antibodies vary in host species (rabbit, mouse) and clonality (monoclonal/polyclonal), requiring validation for specificity due to potential cross-reactivity with homologous receptors. Recent studies using GPRC6A antibodies have clarified its tissue distribution, ligand specificity, and role in metabolic disorders, aiding drug discovery for related diseases.
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