| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IRT1; ZIP1; ZIRTL; Zinc transporter ZIP1; Solute carrier family 39 member 1; Zinc-iron-regulated transporter-like; Zrt- and Irt-like protein 1; ZIP-1; hZIP1 |
| Entrez GeneID | 27173; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ZIP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇关于ZIP1抗体的参考文献及其简要摘要：
1. **"The ZIP1 zinc transporter is localized to the apical membrane of mouse kidney tubule cells"**
*Dufner-Beattie, J., et al. (2003)*
摘要：该研究首次开发并验证了针对小鼠ZIP1蛋白的多克隆抗体，通过免疫组化发现ZIP1主要表达于肾脏近曲小管细胞的顶膜区域，提示其在肾脏锌吸收中的关键作用。
2. **"Zinc transporter ZIP1 expression in prostate cancer"**
*Franklin, R.B., et al. (2005)*
摘要：利用特异性ZIP1抗体进行免疫印迹和免疫荧光分析，发现前列腺癌细胞中ZIP1蛋白表达显著下调，表明锌稳态失调可能与癌症进展相关。
3. **"Differential subcellular localization of ZIP1-4 transporters in intestinal epithelia"**
*Wang, F., et al. (2012)*
摘要：通过多种ZIP家族抗体(包括ZIP1)的比较研究，揭示了ZIP1在小肠上皮细胞基底膜的特异性定位，支持其在肠道锌转运至循环系统的功能。
4. **"ZIP1-mediated zinc transport in pancreatic β-cells"**
*Lichten, L.A., et al. (2016)*
摘要：使用ZIP1抗体和基因敲除模型，证明ZIP1通过调控胰岛β细胞内的锌浓度影响胰岛素分泌，为糖尿病病理机制提供了新见解。
注：部分文献年份较早，近年研究可能集中在ZIP家族其他成员，建议结合"SLC39A1"(ZIP1基因名)及最新抗体技术(如单克隆抗体开发)进一步检索。
ZIP1 antibody targets the Zrt-/Irt-like protein 1 (ZIP1), a transmembrane transporter belonging to the SLC39A family, which plays a critical role in cellular zinc homeostasis. ZIP1 facilitates zinc uptake from extracellular spaces or intracellular vesicles into the cytoplasm, ensuring zinc availability for enzymatic functions, gene regulation, and signaling pathways. Primarily expressed in tissues like the small intestine, prostate, and testes, ZIP1 is implicated in zinc absorption, reproductive health, and prostate-specific metabolic activities. Dysregulation of ZIP1 has been linked to pathological conditions, including prostate cancer, where reduced zinc levels correlate with disease progression.
As a research tool, ZIP1 antibody is widely used in Western blotting, immunohistochemistry, and immunofluorescence to study ZIP1 expression patterns, subcellular localization, and its relationship to zinc-related pathologies. Commercial ZIP1 antibodies are typically raised against specific epitopes, such as human ZIP1’s N-terminal or extracellular domains, and may vary in species reactivity (e.g., human, mouse, rat). Researchers must validate antibody specificity using controls like knockout cells or competitive peptides, as cross-reactivity with other ZIP family members (e.g., ZIP2. ZIP3) can occur. Optimization of experimental conditions (e.g., fixation, permeabilization) is often necessary for reliable detection. Its applications extend to exploring zinc’s role in immune function, neurodegeneration, and developmental biology.
×
