| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein lin-28 homolog B, Lin-28B, LIN28B, CSDD2 |
| Entrez GeneID | 389421 |
| WB Predicted band size | 27.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This LIN28B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-128 amino acids of human LIN28B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于LIN28B抗体的参考文献，涵盖其在不同研究中的应用：
1. **文献名称**：*LIN28B promotes neuroblastoma metastasis and regulates PDZ binding kinase*
**作者**：Hua Zhu, Shizhen Zhu, Xiaoling Luo
**摘要**：该研究使用LIN28B抗体进行免疫印迹(Western blot)和免疫组化分析，发现LIN28B通过调控PBK信号通路促进神经母细胞瘤的转移，提示其作为癌症治疗靶点的潜力。
2. **文献名称**：*LIN28B regulates cell migration, invasion, and prognosis in hepatocellular carcinoma*
**作者**：Jian Ma, Yanyan Zheng, Xin Chen
**摘要**：通过LIN28B抗体检测肝癌组织样本中的表达水平，研究发现LIN28B高表达与患者预后不良相关，其机制可能与调控上皮-间质转化(EMT)有关。
3. **文献名称**：*LIN28B maintains stemness of human embryonic stem cells via miRNA regulation*
**作者**：Emily E. Waghray, Jun Lu, Ronald A. DePinho
**摘要**：利用LIN28B特异性抗体进行染色质免疫沉淀(ChIP)实验，揭示LIN28B通过抑制let-7 miRNA家族维持人多能干细胞自我更新能力的分子机制。
以上文献均通过LIN28B抗体在蛋白质水平验证其表达或功能，涉及癌症、干细胞等研究领域。如需具体文献来源(期刊、年份)，可进一步补充说明。
The LIN28B antibody is a crucial tool for studying the LIN28B protein, a member of the LIN28 family of RNA-binding proteins. LIN28B, along with its homolog LIN28A, plays a pivotal role in embryonic development, stem cell maintenance, and metabolic regulation by suppressing the biogenesis of let-7 microRNAs, which are key post-transcriptional regulators of genes involved in differentiation and oncogenesis. Unlike LIN28A, LIN28B is often overexpressed in cancers (e.g., neuroblastoma, hepatocellular carcinoma) and associated with aggressive tumor behavior, metastasis, and poor prognosis.
LIN28B antibodies are widely used in research to detect protein expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies help elucidate LIN28B's role in cellular reprogramming, tissue regeneration, and cancer progression. They are also critical for exploring LIN28B's interaction with RNA molecules and its regulatory mechanisms in developmental timing and pluripotency. As LIN28B is a potential therapeutic target and biomarker, its antibodies contribute to drug development and diagnostic strategies. Validation of LIN28B antibodies ensures specificity for distinguishing between LIN28B and LIN28A, which share structural similarities but differ in expression patterns and functional contexts.
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