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Rabbit Polyclonal CNN2(N-term) Antibody

  • 中文名: CNN2 (N-term)抗体
  • 别    名: Calponin-2, Calponin H2, smooth muscle, Neutral calponin, CNN2
货号: IPDX31082
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesCalponin-2, Calponin H2, smooth muscle, Neutral calponin, CNN2
Entrez GeneID1265
WB Predicted band size33.7kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenThis CNN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-36 amino acids from the N-terminal region of human CNN2.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于CNN2(N-term)抗体的3篇参考文献的简要概括,基于公开研究领域整理:

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1. **文献名称**:*"Calponin 2 regulates actin cytoskeleton rearrangement in cell migration"*

**作者**:Smith J, et al.

**摘要**:研究利用CNN2 N端特异性抗体,发现CNN2通过调节肌动蛋白动力学影响细胞迁移,在癌症转移模型中抑制CNN2表达可降低侵袭能力。

2. **文献名称**:*"Characterization of calponin 2 in smooth muscle differentiation"*

**作者**:Zhang L, et al.

**摘要**:通过N端抗体免疫印迹和免疫荧光,证实CNN2在平滑肌分化中的表达上调,并参与维持细胞收缩功能,提示其与血管疾病的潜在关联。

3. **文献名称**:*"A novel antibody targeting the N-terminal domain of CNN2 reveals its role in podosome formation"*

**作者**:Garcia-Rico E, et al.

**摘要**:开发并验证了一种高特异性CNN2 N端抗体,证明CNN2在细胞伪足和黏着斑结构中的定位,为细胞力学研究提供新工具。

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注:以上为示例性内容,实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。建议结合具体研究场景补充抗体应用(如Western blot、免疫组化)及疾病模型等信息。

背景信息

CNN2 (N-term) antibody is a polyclonal antibody specifically designed to detect Calponin 2 (CNN2), a protein encoded by the CNN2 gene in humans. Calponin 2. also known as neutral calponin, is an actin-binding protein involved in regulating cytoskeletal dynamics, smooth muscle contraction, and cell migration. It plays roles in various physiological and pathological processes, including vascular remodeling, inflammation, and cancer metastasis. The antibody targets the N-terminal region of human CNN2. generated using a synthetic peptide immunogen conjugated to KLH, typically derived from residues within the first 100 amino acids. It is validated for applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF), demonstrating specificity for CNN2 in human and mouse samples. Researchers use this antibody to study CNN2 expression patterns in tissues, its interaction with actin filaments, and its functional implications in diseases. Validation often includes knockout cell line controls to confirm minimal cross-reactivity with other calponin isoforms (e.g., CNN1 or CNN3). The observed molecular weight of CNN2 is approximately 36 kDa, though post-translational modifications may cause slight variations (e.g., 38–40 kDa in SDS-PAGE). This tool is essential for exploring CNN2's role in cellular mechanics and disease mechanisms.

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