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Rabbit Polyclonal CRYBA1 Antibody

  • 中文名: CRYBA1抗体
  • 别    名: Beta-crystallin A3, Beta-crystallin A3, isoform A1, Delta4 form, Beta-crystallin A3, isoform A1, Delta7 form, Beta-crystallin A3, isoform A1, Delta8 form, CRYBA1, CRYB1
货号: IPDX31075
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 1/1000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/500 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesBeta-crystallin A3, Beta-crystallin A3, isoform A1, Delta4 form, Beta-crystallin A3, isoform A1, Delta7 form, Beta-crystallin A3, isoform A1, Delta8 form, CRYBA1, CRYB1
Entrez GeneID1411
WB Predicted band size25.2kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse
ImmunogenThis CRYBA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human CRYBA1.
FormulationPurified antibody in PBS with 0.05% sodium azide.

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参考文献

以下是关于CRYBA1抗体的3篇参考文献,按文献名称、作者及摘要内容概括整理:

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1. **文献名称**:*CRYBA1 mutations leading to autosomal dominant congenital cataract in a Chinese family*

**作者**:Li J, et al.

**摘要**:该研究利用CRYBA1特异性抗体进行免疫荧光和Western blot分析,发现一个中国家族中CRYBA1基因的错义突变(R21C)导致先天性白内障,突变蛋白在晶状体上皮细胞中异常聚集,干扰细胞透明度。

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2. **文献名称**:*Developmental expression patterns of crystallin genes in murine lens*

**作者**:Smith SB, et al.

**摘要**:通过CRYBA1抗体标记小鼠胚胎及成年晶状体组织,揭示CRYBA1蛋白在发育早期的强表达,并随年龄增长逐渐下调,提示其在晶状体纤维细胞分化中的关键作用。

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3. **文献名称**:*Antibody-based proteomics for cataract-associated protein profiling*

**作者**:Kumar M, et al.

**摘要**:研究采用CRYBA1抗体结合质谱技术,分析白内障患者晶状体蛋白质组变化,发现CRYBA1表达水平显著降低,并与α-晶状体蛋白的互作异常相关,可能参与疾病进展。

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**备注**:以上文献为示例,实际文献需通过PubMed或Web of Science等数据库检索确认。若需具体文章,建议使用关键词“CRYBA1 antibody”+“cataract”或“lens”进一步筛选。

背景信息

The CRYBA1 antibody is a crucial tool for studying βA1-crystallin, a protein encoded by the CRYBA1 gene, which belongs to the crystallin family essential for maintaining lens transparency and refractive properties in the eye. As a major structural component of the vertebrate eye lens, βA1-crystallin forms oligomers with other crystallins to ensure optical clarity. Mutations in CRYBA1 are linked to congenital cataracts, making this protein a key focus in ophthalmology research. CRYBA1 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect protein expression, localization, and abundance in lens tissues or experimental models. These antibodies help investigate disease mechanisms, particularly in cataract formation, and explore potential therapeutic targets. Recent studies also suggest CRYBA1's involvement in non-lens tissues, including retinal neurons and certain tumors, broadening its research applications. As a highly conserved protein across species, CRYBA1 antibodies often demonstrate cross-reactivity in human, mouse, and rat samples, facilitating comparative studies. Proper validation of antibody specificity through knockout controls remains critical, given the structural similarities among crystallin family members.

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