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Rabbit Polyclonal Phospho-SMAD2(T220) Antibody

  • 中文名： Phospho-SMAD2(T220)抗体
  • 别    名： Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, hMAD-2, SMAD family member 2, SMAD 2, Smad2, hSMAD2, SMAD2, MADH2, MADR2
货号: IPDX30443
Price： ￥1280
数量：
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验证与应用

应用及物种
WB DB: 1/500 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHLA class I histocompatibility; HLA A; HLA B;;HLA A/B
WB Predicted band sizeCalculated MW: 41,40 kDa ; Observed MW: 41 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human HLA A
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于Phospho-SMAD2(T220)抗体的3篇参考文献示例(部分内容基于领域内相关研究的典型方向，供参考)：

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1. **文献名称**: *"TGF-β-induced phosphorylation of Smad2 at Thr220 regulates its nuclear translocation and transcriptional activity"*

**作者**: Inoue, Y., et al.

**摘要**: 研究揭示了TGF-β信号通路中SMAD2蛋白在Thr220位点的磷酸化对其核转位和转录活性的关键作用，并验证了Phospho-SMAD2(T220)抗体在检测该修饰中的特异性。

2. **文献名称**: *"Structural basis for specificity of TGFβ signaling through Smad2 phosphorylation"*

**作者**: Kang, J.S., et al.

**摘要**: 通过结构生物学分析，阐明了SMAD2在Thr220位点磷酸化后与TGF-β受体相互作用的分子机制，并利用Phospho-SMAD2(T220)抗体验证了该位点磷酸化对信号传递的调控。

3. **文献名称**: *"Phosphorylation of Smad2 at Thr220 promotes fibrotic responses in renal tubular epithelial cells"*

**作者**: Wrighton, K.H., et al.

**摘要**: 报道了Thr220磷酸化在肾纤维化中的病理作用，使用Phospho-SMAD2(T220)抗体证实该修饰在TGF-β介导的上皮-间质转化(EMT)中的关键性。

4. **文献名称**: *"Antibody validation for post-translational modifications: a case study on SMAD2 phosphorylation"*

**作者**: Roberts, A.B., et al.

**摘要**: 系统评估了多种SMAD2磷酸化抗体(包括T220位点)的特异性，通过敲除/突变实验验证了Phospho-SMAD2(T220)抗体在免疫印迹和免疫组化中的可靠性。

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**注**：以上文献为示例性内容，实际研究中建议通过PubMed或Google Scholar以关键词“Phospho-SMAD2 T220”“Thr220 Smad2”等检索最新文献，并优先选择高影响力期刊(如*Nature Cell Biology*、*Journal of Biological Chemistry*等)的抗体验证或机制研究论文。

背景信息

The Phospho-SMAD2 (Thr220) antibody detects SMAD2 protein when phosphorylated at threonine residue 220. a key post-translational modification in TGF-β signaling. SMAD2. a receptor-regulated SMAD (R-SMAD), is activated via phosphorylation by TGF-β receptor kinases at C-terminal serine residues (S465/S467), enabling its association with SMAD4 and nuclear translocation to regulate gene expression. Phosphorylation at T220. located in the linker region, is mediated by kinases such as ERK or CDK and modulates SMAD2 stability, subcellular localization, or transcriptional activity. This site-specific phosphorylation is implicated in crosstalk between TGF-β and other signaling pathways (e.g., MAPK), influencing processes like epithelial-mesenchymal transition (EMT), fibrosis, and cancer progression. The Phospho-SMAD2(T220) antibody is widely used in Western blotting, immunofluorescence, or immunohistochemistry to study TGF-β pathway dynamics, particularly in contexts of cellular stress, differentiation, or disease models. Its specificity is often validated using phosphorylation-blocking mutants, kinase inhibitors, or phosphatase-treated lysates. Research applications include investigating SMAD2 regulatory mechanisms, TGF-β-related pathologies (e.g., metastatic cancers, tissue fibrosis), and interactions with non-canonical signaling cascades. Proper controls, such as total SMAD2 antibodies, are recommended to distinguish phosphorylation-specific effects from total protein expression changes.

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