| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Calmegin, CLGN |
| Entrez GeneID | 1047 |
| WB Predicted band size | 70.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CLGN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 309-336 amino acids from the Central region of human CLGN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于CLGN抗体的3篇参考文献的模拟示例(注：内容为虚构，仅供格式参考)：
---
1. **文献名称**: *Calmegin (CLGN) as a critical chaperone in sperm-egg interaction: Antibody-based functional analysis*
**作者**: Ikawa M, et al.
**摘要**: 本研究利用CLGN特异性抗体，揭示了Calmegin在小鼠精子-卵子识别中的关键作用。通过免疫荧光和Western blot分析，证实CLGN缺失导致精子表面蛋白折叠异常，阻碍受精能力，为男性不育机制提供了新见解。
2. **文献名称**: *CLGN as a novel cancer/testis antigen: Immunohistochemical profiling using monoclonal antibodies*
**作者**: Hörmann A, et al.
**摘要**: 研究开发了高特异性抗CLGN单克隆抗体，并用于检测其在多种癌症组织中的表达。结果显示CLGN在部分肺癌和乳腺癌中异常表达，提示其作为潜在免疫治疗靶点的价值。
3. **文献名称**: *Generation and validation of anti-CLGN antibodies for co-immunoprecipitation studies in spermatogenesis*
**作者**: Nakamura Y, et al.
**摘要**: 报道了一种新型兔源多克隆抗体的制备与验证，该抗体能高效结合CLGN并用于共免疫沉淀实验，揭示了其与ADAM3等精子发生相关蛋白的相互作用网络。
---
*注：上述文献为示例性内容，实际研究中请通过PubMed或Google Scholar检索真实文献。*
CLGN (Calmegin) antibodies are immunological tools used to study the expression and function of calmegin, a testis-specific protein belonging to the chaperone family. Calmegen is primarily expressed during spermatogenesis, where it plays a critical role in sperm-egg adhesion and male fertility. It is structurally related to heat shock proteins and functions as a molecular chaperone, assisting in the folding and quality control of proteins involved in germ cell development. Research on CLGN gained momentum due to its association with male infertility, as knockout mouse models demonstrated impaired sperm-zona pellucida binding and sterility.
CLGN antibodies are widely employed in reproductive biology to detect calmegin expression in testicular tissues or sperm cells via techniques like Western blot, immunohistochemistry, or immunofluorescence. These antibodies help elucidate calmegin's spatiotemporal expression patterns during spermatogenesis and its interactions with other fertility-related molecules, such as ADAM3. Additionally, they serve as biomarkers in clinical studies exploring genetic causes of human infertility. Commercially available CLGN antibodies are typically raised in rabbits or mice, targeting specific epitopes of the protein. Validation includes reactivity assays using CLGN-deficient samples to confirm specificity. Despite their niche application, these antibodies remain vital for advancing understanding of sperm maturation mechanisms and developing diagnostic or therapeutic strategies for infertility.
×
