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产品描述：人预测humanin肽(MT-RNR2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MT-RNR2含量。MT-RNR2 是线粒体核糖体 RNA 2 基因。它与线粒体功能密切相关，在线粒体蛋白质合成中发挥重要作用。研究发现其突变可能和多种疾病如耳聋等相关，机制可能涉及影响线粒体蛋白质翻译过程，干扰细胞能量代谢，为疾病诊断和治疗提供研究方向。试剂盒检测范围为28 pg/mL-1800 pg/mL，适用于探究疾病模型中humanin的表达调控机制、评估药物或基因干预效果，以及线粒体功能相关的基础研究；为神经科学、代谢疾病及衰老研究领域提供高效解决方案本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MT-RNR2 ELISA Kit; HN ELISA Kit; Humanin ELISA Kit; Humanin mitochondrial ELISA Kit; HNM ELISA Kit
缩写：MT-RNR2
Uniprot No.：Q8IVG9
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MT-RNR2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 100 | ||||
| Range % | 92-105 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:20 | Average % | 97 | ||||
| Range % | 92-106 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human MT-RNR2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-99 | ||||
| EDTA plasma (n=4) | 95 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18002.1202.1632.1422.0079001.6751.6051.6401.5054501.1581.1681.1631.0282250.7410.7800.7610.626112.50.3520.3830.3680.23356.250.2310.2410.2360.101280.1780.1860.1820.04700.1320.1380.135 | ||||||
货期：3-5 working days
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