| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BRE 1B; BRE1 B; BRE1B; RBP95; Rnf40; STARING;;RNF40 |
| WB Predicted band size | Calculated MW: 114 kDa ; Observed MW: 130 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human RNF40 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇与RNF40抗体相关的示例文献(内容为模拟概括，供参考)：
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1. **文献名称**：*RNF40 mediates histone H2B ubiquitination and transcriptional regulation in leukemia*
**作者**：Smith et al.
**摘要**：本研究揭示RNF40通过催化组蛋白H2B的单泛素化(H2Bub1)调控白血病细胞分化。利用RNF40特异性抗体进行ChIP-seq和免疫沉淀，发现其缺失导致关键致癌基因的转录异常，提示其在血液肿瘤中的表观遗传调控作用。
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2. **文献名称**：*RNF40-dependent DNA repair pathway promotes genomic stability*
**作者**：Chen & Wang
**摘要**：通过RNF40抗体敲低实验及Western blot分析，证实RNF40在DNA双链断裂修复中不可或缺。研究发现，RNF40通过泛素化修饰招募BRCA1复合体，维持基因组稳定性，为癌症治疗提供潜在靶点。
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3. **文献名称**：*Antibody-based profiling of RNF40 in triple-negative breast cancer*
**作者**：Gupta et al.
**摘要**：采用RNF40抗体对乳腺癌组织进行免疫组化分析，发现RNF40在三阴性乳腺癌中高表达且与预后不良相关。体外实验显示，抑制RNF40可降低肿瘤细胞侵袭性，表明其作为生物标志物的潜力。
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注：上述文献为示例，实际引用需根据具体研究检索数据库(如PubMed)。若需真实文献，建议以“RNF40 antibody” + “ubiquitination”/“cancer”等关键词搜索近年论文。
The RNF40 antibody is a research tool targeting the RNF40 protein, a key component of the E3 ubiquitin ligase complex critical for histone H2B monoubiquitination (H2BK120ub1). RNF40. along with RNF20. forms the heterodimeric core of the complex, which regulates chromatin remodeling, transcriptional elongation, and DNA damage repair. This post-translational modification is essential for epigenetic regulation, influencing processes like cell differentiation, apoptosis, and oncogenesis. Dysregulation of RNF40 has been linked to cancer progression, inflammatory diseases, and neurological disorders, making it a focus of therapeutic exploration.
RNF40 antibodies are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to study protein expression, localization, and interaction partners. They help elucidate RNF40's role in modulating NF-κB signaling, maintaining genomic stability, and coordinating with other ubiquitination machinery like BRCA1/BARD1. Commercial antibodies vary in specificity, often validated for human/mouse models, with applications in both basic research and drug development. Recent studies highlight RNF40's tumor-suppressive or oncogenic duality in different cancers, emphasizing the need for reliable antibodies to dissect context-dependent functions. Proper validation via knockout controls remains crucial due to potential cross-reactivity with homologous E3 ligases.
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