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产品描述：人甲硫氨酸合成酶(MTR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MTR含量。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究场景，例如：通过检测细胞模型中MTR活性变化探究代谢调控机制，分析动物疾病模型组织匀浆的酶表达水平，或评估生物样本中同型半胱氨酸代谢通路相关标志物。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MTRMethionine synthase ELISA Kit; EC 2.1.1.13 ELISA Kit; 5-methyltetrahydrofolate--homocysteine methyltransferase ELISA Kit; Vitamin-B12 dependent methionine synthase ELISA Kit; MS ELISA Kit
缩写：MTR
Uniprot No.：Q99707
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 98 | |||||
| Range % | 94-103 | ||||||
| 1:2 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 91-99 | ||||||
| 1:8 | Average % | 87 | |||||
| Range % | 83-92 | ||||||
回收率：
| The recovery of human MTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 108 | 104-112 | |||||
| EDTA plasma (n=4) | 85 | 80-90 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected102.8642.7652.8152.65352.2452.1412.1932.0312.51.3541.4051.3801.2181.250.8150.8310.8230.6610.6250.5400.5520.5460.3840.3120.3510.3420.3470.1850.1560.2520.2490.2510.08900.1610.1630.162 | |||||||
货期：3-5 working days
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