首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒

Human mammalian target of rapamyein,mTOR ELISA Kit

  • 中文名: 人雷帕霉素靶蛋白(mTOR)ELISA Kit
货号: IPD-E09038h
Price: ¥2500
数量:
大包装询价

产品详情

  • 产品描述:人雷帕霉素靶蛋白(mTOR)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的mTOR含量。mTOR是一种关键的蛋白质靶点,在细胞生长、增殖、代谢等过程中起重要作用。其异常激活与多种疾病如癌症、代谢疾病等密切相关。研究机制主要聚焦其上下游信号通路,探索调控其活性的方式,为开发针对性治疗药物提供理论依据。试剂盒检测范围为1.56 pg/mL-100 pg/mL,适用于科研实验中精确分析mTOR蛋白表达水平;为疾病机制探索、药物靶点验证及基础医学研究提供可靠数据支持;尤其适用于体外实验中评估药物干预、基因修饰或病理模型对mTOR信号通路的影响;满足实验室对哺乳动物样本中低浓度mTOR蛋白的定量检测需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。

  • 别名:dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) ELISA Kit; FK506 binding protein 12 rapamycin associated protein 1 ELISA Kit; FK506 binding protein 12 rapamycin associated protein 2 ELISA Kit; FK506 binding protein 12 rapamycin complex associated protein 1 ELISA Kit; FK506-binding protein 12-rapamycin complex-associated protein 1 ELISA Kit; FKBP rapamycin associated protein ELISA Kit; FKBP12 rapamycin complex associated protein ELISA Kit; FKBP12-rapamycin complex-associated protein 1 ELISA Kit; FKBP12-rapamycin complex-associated protein ELISA Kit; FLJ44809 ELISA Kit; FRAP ELISA Kit; FRAP1 ELISA Kit; FRAP2 ELISA Kit; Mammalian target of rapamycin ELISA Kit; Mechanistic target of rapamycin ELISA Kit; mTOR ELISA Kit; MTOR_HUMAN ELISA Kit; OTTHUMP00000001983 ELISA Kit; RAFT1 ELISA Kit; Rapamycin and FKBP12 target 1 ELISA Kit; Rapamycin associated protein FRAP2 ELISA Kit; Rapamycin target protein 1 ELISA Kit; Rapamycin target protein ELISA Kit; RAPT1 ELISA Kit; Serine/threonine-protein kinase mTOR ELISA Kit

  • 缩写:mTOR

  • Uniprot No.:P42345

  • 种属:Homo sapiens (Human)

  • 样本类型:serum, plasma, tissue homogenates

  • 检测范围:1.56 pg/mL-100 pg/mL

  • 灵敏度:0.39 pg/mL

  • 反应时间:1-5h

  • 样本体积:50-100ul

  • 检测波长:450 nm

  • 研究领域:Cell Biology

  • 测定原理:quantitative

  • 测定方法:Sandwich

  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%


    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%


    Three samples of known concentration were tested in twenty assays to assess.








  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of human mTOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

    SampleSerum(n=4)
    1:100Average %86
    Range %80-92
    1:200Average %98
    Range %91-105
    1:400Average %101
    Range %92-110
    1:800Average %93
    Range %86-98
  • 回收率:

    The recovery of human mTOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5)9389-98
    EDTA plasma (n=4)9790-100














  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected1002.6752.6662.6712.546502.3122.1892.2512.126251.7091.6631.6861.56112.51.0470.9891.0180.8936.250.6170.6030.6100.4853.120.4290.4120.4210.2961.560.2380.2290.2340.10900.1290.1210.125 






  • 货期:3-5 working days

客户数据及评论

折叠内容

大包装询价

×