| WB | 咨询技术 | Human,Mouse,Rat |
| IF | ChIP:1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H3.1, Histone H3, HIST1H3A;;Acetyl-Histone H3 (K24) |
| WB Predicted band size | 15 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Histone H3.1 around the acetylation site of K24 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及Histone H3(acetylK23)抗体的参考文献及其摘要概括：
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1. **文献名称**：*"Site-Specific Acetylation of Histone H3K23 Promotes DNA Repair"*
**作者**：Tjeertes et al.
**摘要**：研究揭示H3K23乙酰化在DNA损伤修复中的关键作用，通过免疫荧光和ChIP实验证实该修饰促进同源重组修复通路，且依赖Tip60乙酰转移酶活性。
2. **文献名称**：*"Dynamic Acetylation of Histone H3K23 during the Cell Cycle"*
**作者**：Shibata et al.
**摘要**：利用H3K23ac特异性抗体追踪细胞周期中该修饰的动态变化，发现其在S期富集于复制起点，可能通过协调染色质复制与转录活性维持基因组稳定性。
3. **文献名称**：*"H3K23ac Modulates Enhancer Activity in Cancer Stem Cells"*
**作者**：Wang et al.
**摘要**：通过ChIP-seq和CRISPR筛选，发现H3K23ac在胶质母细胞瘤干细胞中特异性富集于致癌基因增强子区域，靶向抑制其修饰酶可降低肿瘤干性表型。
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以上研究均使用H3K23ac特异性抗体进行表观遗传学分析，涵盖DNA修复、细胞周期调控及肿瘤生物学领域。如需具体DOI或期刊信息可进一步补充。
The Histone H3 (acetylK23) antibody is a specialized tool used to detect acetylation at lysine 23 (K23) on histone H3. a post-translational modification critical for epigenetic regulation. Histones are core proteins around which DNA wraps to form nucleosomes, and their post-translational modifications, such as acetylation, influence chromatin structure and gene expression. Acetylation of histones, including H3K23. reduces the positive charge of lysine residues, weakening their interaction with negatively charged DNA. This loosens chromatin, promoting transcriptional activation by allowing access to transcription factors and regulatory proteins.
The acetylation of H3K23 is associated with active transcription, DNA repair, and cell cycle progression. It is dynamically regulated by histone acetyltransferases (HATs) and deacetylases (HDACs). Aberrant H3K23 acetylation has been implicated in diseases such as cancer, neurodegenerative disorders, and developmental defects, making its study vital for understanding disease mechanisms.
The Histone H3 (acetylK23) antibody is widely used in techniques like chromatin immunoprecipitation (ChIP), immunofluorescence, and Western blotting to map epigenetic changes, study gene regulation, or assess therapeutic responses targeting epigenetic modifiers. Its specificity for the acetylated K23 residue ensures accurate detection, distinguishing it from other acetylation sites on histone H3 (e.g., K9. K14. K18). Researchers rely on this antibody to explore links between chromatin dynamics, cellular processes, and disease pathology, highlighting its importance in epigenetics and molecular biology research.
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