首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人激肽释放酶1(KLK 1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KLK1含量。KLK1是一种靶点。它属于激肽释放酶家族，参与激肽生成等生理过程。研究发现其在心血管、肾脏等系统中发挥重要作用，影响血压调节、组织修复与保护等。对KLK1作用机制的深入研究，或为相关疾病诊治提供新思路。试剂盒检测范围为0.78 ng/mL-50 ng/mL，本试剂盒适用于基础科研中KLK1的定量分析，可为高血压、动脉粥样硬化或组织修复相关分子机制探索提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Glandular kallikrein 1 ELISA Kit; hK 1 ELISA Kit; hK1 ELISA Kit; Kallikrein serine protease 1 ELISA Kit; Kallikrein-1 ELISA Kit; Kidney/pancreas/salivary gland kallikrein ELISA Kit; Klk 6 ELISA Kit; KLK1 ELISA Kit; KLK1_HUMAN ELISA Kit; Klk6 ELISA Kit; KLKR ELISA Kit; Tissue kallikrein ELISA Kit
缩写：KLK1
Uniprot No.：P06870
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 85-103 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 83-98 | |||||
回收率：
| The recovery of human KLK 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-101 | ||||
| EDTA plasma (n=4) | 89 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.3052.3252.3152.109251.6291.6571.6431.43712.51.1241.1391.1320.9266.250.6960.6830.6900.4843.120.5020.4950.4990.2931.560.3550.3420.3490.1430.780.2840.3000.2920.08600.2050.2070.206 | ||||||
货期：3-5 working days
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