首页 / 产品 / 抗体 / 一抗

Rabbit Monoclonal MAD1 Antibody

  • 中文名: MAD1抗体
  • 别    名: hMAD1; HsMAD1; MAD1; MAD1L1; PIG9; TP53I9; TXBP181;;MAD1
货号: IPDX18184
Price: ¥1280
数量:
大包装询价

验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 咨询技术 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliaseshMAD1; HsMAD1; MAD1; MAD1L1; PIG9; TP53I9; TXBP181;;MAD1
WB Predicted band sizeCalculated MW: 83 kDa ; Observed MW: 80 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human MAD1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

+ +

参考文献

以下是关于MAD1抗体的3篇示例文献及其摘要概述(注:以下文献为示例,非真实存在):

1. **文献名称**:*MAD1 Antibody Validation for Mitotic Checkpoint Studies*

**作者**:Chen L, et al.

**摘要**:本研究验证了一种高特异性的MAD1抗体,通过CRISPR敲除细胞系和免疫印迹分析,证实其在检测有丝分裂检查点中MAD1蛋白表达的可靠性,并应用于活细胞成像观察MAD1的纺锤体定位。

2. **文献名称**:*Aberrant MAD1 Expression in Colorectal Cancer Progression*

**作者**:Wang R, et al.

**摘要**:利用MAD1抗体对结直肠癌组织进行免疫组化分析,发现MAD1表达水平降低与染色体不稳定性和肿瘤转移显著相关,提示其作为潜在预后标志物的可能性。

3. **文献名称**:*Dynamic Localization of MAD1 During Cell Division*

**作者**:Johnson M, et al.

**摘要**:通过MAD1抗体的免疫荧光染色,实时追踪了MAD1蛋白在有丝分裂不同阶段的定位变化,揭示其与微管结合蛋白的相互作用对检查点激活的关键调控机制。

---

如需真实文献,建议通过PubMed或Google Scholar检索关键词“MAD1 antibody application”或“MAD1 antibody validation”获取最新研究。

背景信息

The MAD1 (Mitotic Arrest Deficient 1) protein is a critical component of the spindle assembly checkpoint (SAC), a surveillance mechanism that ensures accurate chromosome segregation during mitosis. MAD1. along with MAD2. forms a heterocomplex that detects unattached or improperly attached kinetochores, delaying anaphase onset until all chromosomes achieve proper microtubule attachment. This checkpoint prevents aneuploidy, a hallmark of genomic instability linked to cancer and developmental disorders. Dysregulation of MAD1 disrupts SAC signaling, leading to premature mitotic exit and chromosomal missegregation.

MAD1 antibodies are essential tools for studying SAC function, protein localization, and interactions in cell cycle regulation. They are widely used in techniques like immunofluorescence, Western blotting, and co-immunoprecipitation to investigate MAD1's role in mitosis, cancer biology, and response to anti-mitotic therapies. Research has also explored MAD1 mutations or expression anomalies in tumors, suggesting its potential as a diagnostic or prognostic marker. Additionally, MAD1 antibodies aid in understanding crosstalk between SAC proteins and other pathways, such as DNA damage repair, offering insights into therapeutic strategies targeting cell cycle checkpoints in cancer treatment.

客户数据及评论

折叠内容

大包装询价

×