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产品描述:人TGF-β诱导早期基因1(TIEG1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KLF10含量。KLF10是一种重要靶点。它属于Krüppel样因子家族,参与细胞增殖、分化、凋亡等多种生理过程。研究机制上,其通过与特定DNA序列结合调控下游基因表达,在癌症、心血管等疾病发生发展中扮演重要角色,是潜在治疗靶点。试剂盒检测范围为34.38 pg/mL-2200 pg/mL,适用于体外培养细胞模型、动物实验组织样本或体液样本中TIEG1蛋白的动态监测,为探索其在细胞信号转导、基因调控网络及疾病机制中的作用提供定量分析工具,满足基础科研与转化医学研究的多样化需求。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Early growth response; alpha ELISA Kit; Early growth response; alpha like ELISA Kit; EGR A ELISA Kit; EGR alpha ELISA Kit; EGR(A) ELISA Kit; EGR-alpha ELISA Kit; EGRA ELISA Kit; Egral ELISA Kit; EGRalpha ELISA Kit; Gdnfif ELISA Kit; KLF 10 ELISA Kit; KLF10 ELISA Kit; KLF10_HUMAN ELISA Kit; Krueppel like factor 10 ELISA Kit; Krueppel like factor10 ELISA Kit; Krueppel-like factor 10 ELISA Kit; Kruppel like factor 10 ELISA Kit; mGIF ELISA Kit; TGF-beta- inducible early gene ELISA Kit; TGFB inducible early growth response 1 ELISA Kit; TGFB inducible early growth response ELISA Kit; TGFB inducible early growth response protein 1 ELISA Kit; TGFB-inducible early growth response protein 1 ELISA Kit; TIEG 1 ELISA Kit; TIEG ELISA Kit; TIEG-1 ELISA Kit; TIEG1 ELISA Kit; Transforming growth factor beta inducible early growth response ELISA Kit; Transforming growth factor beta inducible early growth response protein 1 ELISA Kit; Transforming growth factor-beta-inducible early growth response protein 1 ELISA Kit; Zinc finger transcription factor TIEG ELISA Kit
缩写:KLF10
Uniprot No.:Q13118
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:34.38 pg/mL-2200 pg/mL
灵敏度:8.6 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cancer
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human TIEG1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 85 | |||||
Range % | 81-89 | ||||||
1:2 | Average % | 93 | |||||
Range % | 90-96 | ||||||
1:4 | Average % | 84 | |||||
Range % | 81-86 | ||||||
1:8 | Average % | 95 | |||||
Range % | 91-99 |
回收率:
The recovery of human TIEG1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 103 | 100-105 | |||||
EDTA plasma (n=4) | 98 | 94-102 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected22002.4352.4312.4332.31011001.7081.6871.6981.5755501.0201.0001.0100.8872750.5330.5700.5520.429137.50.3610.3580.3600.23768.750.2910.2870.2890.16634.380.2030.2120.2080.08500.1220.1240.123 |
货期:3-5 working days
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