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产品描述：人Krueppel样因子4(KLF4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的KLF4含量。KLF4是一个重要靶点。它在细胞增殖、分化、凋亡等多种生理过程中发挥关键作用。其研究机制主要围绕调控下游基因表达来实现细胞功能的调节。在肿瘤、心血管等疾病领域的研究不断深入，有望为相关疾病的治疗提供新靶点和策略。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，适用于基础科研中对KLF4表达水平的动态监测，如干细胞分化研究、肿瘤微环境分析、再生医学相关机制探索等场景；为分子机制研究、药物筛选模型验证等提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Endothelial Kruppel like zinc finger protein ELISA Kit; Epithelial zinc finger protein EZF ELISA Kit; EZF ELISA Kit; GKLF ELISA Kit; gut Kruppel-like factor ELISA Kit; Gut-enriched krueppel-like factor ELISA Kit; KLF ELISA Kit; KLF4 ELISA Kit; KLF4_HUMAN ELISA Kit; Krueppel-like factor 4 ELISA Kit; Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor) ELISA Kit; Kruppel like factor 4 (gut) ELISA Kit
缩写：KLF4
Uniprot No.：O43474
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human KLF4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 85-92 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 90-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human KLF4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 83 | 80-86 | ||||
| EDTA plasma (n=4) | 98 | 95-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 12002.4782.4982.4882.304 6002.0152.1362.0761.892 3001.4121.4651.4391.255 1500.7680.7580.7630.579 750.4980.4720.4850.301 37.50.3680.3550.3620.178 18.750.2710.2820.2770.093 00.1830.1850.184 | ||||||
货期：3-5 working days
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