| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | pADPRT-2; hPARP-2; ADPRT2; ADPRTL2;;PARP 2 |
| WB Predicted band size | 66 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human PARP 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于PARP2抗体的代表性文献示例(注：以下内容为模拟虚构，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*"Development and Validation of a Novel PARP2-Specific Antibody for Immunoblotting and Immunofluorescence"*
**作者**：Smith A, et al. (2021)
**摘要**：本研究开发了一种高特异性的兔源多克隆PARP2抗体，验证了其在Western blot和免疫荧光中的应用。通过敲除细胞系证实抗体无交叉反应性，为PARP2亚细胞定位研究提供工具。
2. **文献名称**：*"Comparative Analysis of PARP Family Antibodies Reveals Distinct Roles of PARP1 and PARP2 in DNA Damage Repair"*
**作者**：Zhang L, et al. (2019)
**摘要**：文章系统比较了多种PARP家族抗体(包括PARP2)的特异性，发现某些商用抗体存在交叉反应。通过CRISPR-Cas9验证，明确了PARP2在DNA单链断裂修复中的独特功能。
3. **文献名称**：*"PARP2 Antibody-Based Screening Identifies Its Interaction with RNA Polymerase II in Transcription Regulation"*
**作者**：Kim J, et al. (2017)
**摘要**：利用特异性PARP2抗体进行免疫共沉淀(Co-IP)，首次发现PARP2与RNA聚合酶II的相互作用，提示其在转录调控中的新功能，拓展了PARP2的非经典作用机制。
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如需实际文献，建议通过 **PubMed** 或 **Google Scholar** 搜索关键词“PARP2 antibody specificity”、“PARP2 antibody application”等获取最新研究。
Poly(ADP-ribose) polymerase 2 (PARP2) is a nuclear enzyme belonging to the PARP family, which plays critical roles in DNA repair, genomic stability, and cellular stress responses. It catalyzes the transfer of ADP-ribose units to target proteins, forming poly(ADP-ribose) (PAR) chains that facilitate DNA damage repair, chromatin remodeling, and transcriptional regulation. PARP2 shares functional overlap with PARP1 but has distinct substrate preferences and structural features, including a unique N-terminal domain. It is implicated in base excision repair (BER), single-strand break repair, and replication stress responses. PARP2 also regulates adipocyte differentiation, inflammatory signaling, and cell death pathways.
PARP2-specific antibodies are essential tools for studying its expression, localization, and molecular interactions. These antibodies enable detection via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Given the structural similarities among PARP family members, high specificity is crucial to avoid cross-reactivity, particularly with PARP1. Researchers use PARP2 antibodies to explore its roles in cancer, neurodegenerative diseases, and metabolic disorders, as well as its involvement in PARP inhibitor resistance. The development of selective PARP2 inhibitors in oncology has further driven demand for reliable antibodies to assess target engagement and therapeutic efficacy. Validated antibodies help clarify PARP2’s non-redundant functions and its potential as a biomarker or therapeutic target in precision medicine.
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