| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Na+/Ca2+ exchanger; NCX1; SLC8A1;;SLC8A1 |
| WB Predicted band size | Calculated MW: 109 kDa ; Observed MW: 108 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human SLC8A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NCX1抗体的3篇参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*"Immunolocalization of the Na+/Ca2+ exchanger in mammalian cardiomyocytes"*
**作者**：K. D. Philipson, A. Y. Nicoll, et al.
**摘要**：研究利用特异性NCX1抗体，通过免疫组化技术定位了钠钙交换体(NCX1)在哺乳动物心肌细胞中的分布，发现其主要位于细胞膜和T小管系统，提示其在调节心肌收缩和钙稳态中的关键作用。
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2. **文献名称**：*"Role of NCX1 in neuronal calcium handling and ischemic brain damage"*
**作者**：T. P. Obata, S. Matsuda, et al.
**摘要**：通过Western blot和免疫荧光技术，使用NCX1抗体证实了该蛋白在神经元中的高表达。实验表明，NCX1在脑缺血期间通过反向模式加剧钙超载，敲低NCX1可显著减少缺血性脑损伤。
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3. **文献名称**：*"Altered expression of Na+/Ca2+ exchanger in hypertension-induced cardiac hypertrophy"*
**作者**：L. G. Perez-Reyes, J. Zhang, et al.
**摘要**：研究利用NCX1抗体检测高血压模型中心肌肥大组织的蛋白表达水平，发现NCX1表达上调与心肌细胞钙信号异常相关，提示其可能成为治疗病理性心脏肥大的靶点。
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如需更多文献或具体细节，可进一步提供研究方向或数据库检索支持。
The Na+/Ca2+ exchanger 1 (NCX1) antibody is a crucial tool for studying the NCX1 protein, a bidirectional ion transporter encoded by the SLC8A1 gene. NCX1 plays a pivotal role in maintaining intracellular calcium homeostasis by exchanging three Na+ ions for one Ca2+ ion across cell membranes. It is highly expressed in excitable tissues, including cardiac muscle, smooth muscle, and neurons, where it regulates calcium efflux during relaxation and contributes to action potential dynamics. Dysregulation of NCX1 has been linked to cardiovascular pathologies (e.g., heart failure, arrhythmias), neurological disorders, and hypertension.
NCX1 antibodies are widely used in research to detect protein expression, localization, and functional changes via techniques like Western blotting, immunohistochemistry, and immunofluorescence. These antibodies are typically raised against specific epitopes of the NCX1 protein, such as intracellular regulatory domains or extracellular loops, and validated for species reactivity (e.g., human, mouse, rat). Monoclonal and polyclonal variants are available, with validation often including knockout controls to confirm specificity. Recent studies also utilize NCX1 antibodies to explore its role in ischemia-reperfusion injury, pharmacological responses, and crosstalk with other calcium-handling proteins like SERCA and RyR. Proper antibody selection depends on experimental goals, as post-translational modifications or tissue-specific splice variants (e.g., NCX1.1. NCX1.3) may affect detection.
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