首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人整合素β-1(ITGB1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ITGB1含量。ITGB1 即整合素β1，在细胞黏附、迁移、增殖和存活等多种生理过程中起关键作用。它与多种疾病发生发展相关，如肿瘤转移、组织纤维化等。研究机制主要聚焦其信号通路激活及与其他分子相互作用，以探索疾病治疗新靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于细胞黏附机制研究、肿瘤微环境分析、组织再生模型等基础科研领域，支持血清/血浆样本直接检测及组织样本匀浆处理后分析，满足实验室对 ITGB1 蛋白表达水平的系统性研究需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ITGB1; FNRB; MDF2; MSK12; Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD antigen CD29
缩写：ITGB1
Uniprot No.：P05556
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ITGB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 86-96 | |||||
| 1:10 | Average % | 89 | ||||
| Range % | 85-94 | |||||
| 1:20 | Average % | 88 | ||||
| Range % | 82-92 | |||||
| 1:40 | Average % | 102 | ||||
| Range % | 98-106 | |||||
回收率：
| The recovery of human ITGB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 102 | 93-105 | ||||
| EDTA plasma (n=4) | 87 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.3612.4692.4152.28420002.0151.9241.9701.83910001.3321.3561.3441.2135000.7410.7320.7370.6062500.4020.4130.4080.2771250.2440.2550.2500.11962.50.1810.1880.1850.05400.1300.1320.131 | ||||||
货期：3-5 working days
×
