| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human SPDL1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
+ +
以下是3篇关于SPDL1抗体的模拟参考文献示例(注：SPDL1为假设性名称，实际文献需根据具体研究领域核实)：
---
1. **文献名称**: "Development of a Monoclonal Antibody Targeting SPDL1 for Mitotic Progression Studies"
**作者**: Zhang L, et al.
**摘要**: 本研究开发了一种高特异性的抗SPDL1单克隆抗体，用于检测细胞有丝分裂过程中SPDL1蛋白的动态定位。通过免疫荧光和Western blot验证，该抗体可特异性识别SPDL1在纺锤体组装检查点(SAC)中的功能，为研究染色体分离异常提供了工具。
2. **文献名称**: "SPDL1 as a Novel Biomarker in Triple-Negative Breast Cancer: Insights from Antibody-Based Profiling"
**作者**: Gupta R, et al.
**摘要**: 利用抗SPDL1抗体对三阴性乳腺癌组织进行免疫组化分析，发现SPDL1高表达与患者预后不良相关。研究提示SPDL1可能通过调控细胞周期蛋白参与肿瘤侵袭性，其抗体可作为潜在诊断标志物。
3. **文献名称**: "Functional Characterization of SPDL1 in Kinetochore-Microtubule Attachment Using CRISPR and Antibody Blockade"
**作者**: Watanabe T, et al.
**摘要**: 通过抗SPDL1抗体阻断实验结合CRISPR技术，揭示了SPDL1在动粒-微管连接中的关键作用。研究发现SPDL1缺失导致染色体排列异常，其抗体可特异性抑制该蛋白功能，为研究染色体不稳定性疾病提供新视角。
---
如需实际文献，建议通过PubMed或Google Scholar以“SPDL1 antibody”或“SPDL1 function”为关键词检索，并核实目标蛋白的标准化名称(如确认是否为**Spindly蛋白**或存在拼写差异)。
**Background of SPDL1 Antibody**
SPDL1 (Spindle Apparatus Coiled-Coil Domain Protein 1) is a critical regulatory protein involved in mitotic processes, particularly in the spindle assembly checkpoint (SAC), which ensures accurate chromosome segregation during cell division. It interacts with key checkpoint proteins like MAD2 and CDC20. playing a role in delaying anaphase until proper microtubule-kinetochore attachments are established. Dysregulation of SPDL1 is linked to genomic instability, aneuploidy, and cancer progression.
Antibodies targeting SPDL1 are essential tools for studying its expression, localization, and function in cellular models. They are widely used in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to investigate SPDL1’s role in mitosis, SAC signaling, and its potential as a biomarker in cancer. Research has shown that SPDL1 overexpression correlates with poor prognosis in certain cancers, making it a candidate for therapeutic targeting.
SPDL1 antibodies also aid in exploring cell cycle defects, mitotic errors, and responses to anti-mitotic drugs. Their specificity and validation are crucial for distinguishing SPDL1 from structurally related proteins and ensuring accurate experimental outcomes. As interest in mitotic regulators grows, SPDL1 antibodies remain pivotal in advancing understanding of cell division mechanics, cancer biology, and targeted therapy development.
×
