| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | insE1 |
| Uniprot No | P0CF66 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-99aa |
| 氨基酸序列 | MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK |
| 预测分子量 | 37.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于insE1重组蛋白的参考文献示例(内容为假设性描述，仅供参考)：
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1. **标题**: *Expression and Purification of Recombinant insE1 in Escherichia coli*
**作者**: Smith A, et al.
**摘要**: 该研究优化了insE1重组蛋白在大肠杆菌中的表达条件，采用His标签纯化技术获得高纯度蛋白，并通过Western blot验证其抗原性，为后续功能研究奠定基础。
2. **标题**: *Structural Characterization of insE1 by Cryo-Electron Microscopy*
**作者**: Chen L, et al.
**摘要**: 通过冷冻电镜技术解析了insE1重组蛋白的三维结构，揭示了其与胰岛素受体结合的关键结构域，为开发靶向药物提供了分子基础。
3. **标题**: *Functional Analysis of insE1 in Glucose Homeostasis*
**作者**: Tanaka K, et al.
**摘要**: 在小鼠模型中验证insE1重组蛋白对血糖调节的作用，发现其可显著改善胰岛素敏感性，提示其在糖尿病治疗中的潜在应用价值。
4. **标题**: *High-Yield Production of insE1 in Baculovirus-Insect Cell System*
**作者**: Müller R, et al.
**摘要**: 利用杆状病毒-昆虫细胞表达系统实现insE1的高效分泌表达，并证明其糖基化修饰对蛋白稳定性和生物活性的重要性。
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注：以上文献为示例，实际研究中请通过学术数据库(如PubMed、Google Scholar)检索真实文献。
**Background of insE1 Recombinant Protein**
The insE1 recombinant protein is a biologically engineered derivative of the insulin E-peptide region, a segment encoded within the insulin gene (INS) in humans. Insulin, a critical hormone for glucose homeostasis, is synthesized as a precursor molecule, proinsulin, which undergoes proteolytic cleavage to release mature insulin and the connecting C-peptide. The E-peptide, though less studied, is a short segment transiently present during proinsulin processing and is hypothesized to play roles in insulin folding, stability, or intracellular trafficking.
insE1 specifically refers to a recombinant variant designed to isolate and characterize the functional or structural properties of this E-peptide domain. Using recombinant DNA technology, the gene sequence encoding the E-peptide is cloned into expression vectors (e.g., bacterial or mammalian systems) to produce high-purity insE1 protein. This enables researchers to study its interactions with insulin receptors, chaperone proteins, or other biomolecules in vitro, bypassing the challenges of isolating the peptide from natural sources.
Interest in insE1 stems from its potential applications in diabetes research, particularly in understanding insulin biosynthesis defects or mutations linked to diabetic disorders. Additionally, recombinant insE1 serves as a tool for developing diagnostic assays or therapeutic agents targeting insulin-related pathways. Its production often includes tags (e.g., His-tag) for simplified purification and tracking, ensuring reproducibility in experimental settings. Recent studies also explore its role in beta-cell biology and its utility as a biomarker for pancreatic function. Overall, insE1 exemplifies how recombinant proteins facilitate mechanistic insights into metabolic diseases while offering translational potential.
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