| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human NFX1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
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以下是关于NFX1抗体的3篇参考文献，内容基于真实研究概括，但文献标题和作者为示例性描述：
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1. **标题**：*NFX1 regulates HPV oncogene expression in cervical cancer cells*
**作者**：Smith A, et al.
**摘要**：研究揭示了NFX1通过结合HPV病毒启动子调控E6/E7癌基因表达的机制。通过NFX1特异性抗体进行染色质免疫沉淀(ChIP)，证实其直接参与转录抑制，可能影响宫颈癌进展。
2. **标题**：*Interaction of NFX1 with MCM7 complex in DNA replication*
**作者**：Lee J, et al.
**摘要**：利用NFX1抗体进行免疫共沉淀(Co-IP)和蛋白质组学分析，发现NFX1与MCM7复合物相互作用，可能调控DNA复制及基因组稳定性，为癌症治疗提供新靶点。
3. **标题**：*Development of a monoclonal antibody targeting NFX1-123 isoform*
**作者**：Wang Y, et al.
**摘要**：报道了一种特异性识别NFX1-123亚型单克隆抗体的开发与验证，通过Western blot和免疫组化证实其在正常与肿瘤组织中的差异表达，支持其在癌症诊断中的应用。
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如需具体文献，建议通过PubMed或Google Scholar检索关键词“NFX1 antibody”、“NFX1 function”等，并筛选涉及抗体实验的研究。
The NFX1 (Nuclear Transcription Factor, X-box Binding 1) protein is a transcription factor involved in regulating gene expression, particularly through interactions with the X-box DNA motif. It exists in two major isoforms, NFX1-123 and NFX1-91. generated via alternative splicing. NFX1-123 contains tandem zinc finger domains critical for its role in transcriptional repression, while NFX1-91 lacks these domains and may function as a dominant-negative regulator. NFX1 is implicated in immune response modulation, cellular differentiation, and viral-host interactions, including pathways linked to human papillomavirus (HPV) infections and cancer progression.
Antibodies targeting NFX1 are essential tools for studying its expression, localization, and molecular interactions. These antibodies, often developed in rabbit or mouse hosts, enable detection via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Specificity is validated using knockout cell lines or recombinant protein controls. Research applications include investigating NFX1's role in telomerase regulation, immune signaling (e.g., NF-κB pathways), and its dual function as a tumor suppressor or oncogene depending on cellular context. Recent studies also explore its interplay with viral oncoproteins, offering insights into therapeutic targets for HPV-associated malignancies.
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