关键信息
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基因名
PHKG1
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简介
PHKG1 is the catalytic subunit of phosphorylase b kinase (PHK), which plays a key role in the neural and hormonal regulation of glycogenolysis (glycogen breakdown) by phosphorylating and activating glycogen phosphorylase. PHKG1 Protein, Human (Active, sf9, GST) is the recombinant human-derived PHKG1, expressed by Sf9 insect cells, with GST labeled tag.
- 应用
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种属
Human
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表达系统
Baculovirus
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标签
GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q16816-1
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表达区间
T2-Y387
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蛋白长度
Partial
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PHKG1 (Phosphorylase Kinase Gamma 1) is a crucial enzyme that plays a significant role in glycogen metabolism, primarily by regulating glycogen phosphorylase through phosphorylation. This enzyme is essential for energy mobilization during muscular contraction and other physiological processes. Mutations or dysregulation of PHKG1 have been implicated in various metabolic disorders, including glycogen storage diseases, which can lead to severe muscle weakness and exercise intolerance. The study of PHKG1 recombinant protein is vital to understanding its structure-function relationships and regulatory mechanisms. By producing and characterizing recombinant PHKG1, researchers can investigate its enzymatic activity, substrate specificity, and interactions with other proteins. Additionally, studying the recombinant protein provides insights into the pathological mechanisms underlying PHKG1-related diseases and paves the way for potential therapeutic interventions. Understanding PHKG1's role in cellular metabolism may also contribute to the development of strategies to enhance muscle performance and treat metabolic disorders. Thus, the research on PHKG1 recombinant protein is essential for both basic science and clinical applications, offering a foundation for future studies aiming to unravel the complexities of glycogen metabolism and its associated diseases.
