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Rabbit Polyclonal IWS1 Antibody

  • 中文名: IWS1抗体
  • 别    名: nan
货号: IPDX12895
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/25-1/100 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human IWS1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于IWS1抗体的虚构参考文献示例(内容基于学术文献常见主题合理虚构,非真实文献):

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1. **文献名称**:IWS1调控RNA聚合酶II转录延伸的分子机制

**作者**:Thompson R. et al.

**摘要**:本研究通过免疫共沉淀(Co-IP)和IWS1特异性抗体验证,揭示了IWS1蛋白通过结合组蛋白修饰酶复合物,调控RNA聚合酶II的转录延伸过程,对基因表达精准性至关重要。

2. **文献名称**:IWS1在乳腺癌细胞迁移中的功能研究

**作者**:Zhang L. et al.

**摘要**:利用IWS1抗体进行免疫荧光染色和Western blot分析,发现IWS1通过调控EMT相关基因的表达促进乳腺癌细胞侵袭,提示其作为潜在治疗靶点的价值。

3. **文献名称**:IWS1与染色质重塑复合物相互作用的结构解析

**作者**:Kumar S. et al.

**摘要**:通过CRISPR-Cas9敲除结合IWS1抗体的ChIP-seq技术,阐明IWS1与Spt6复合物的结构互作域,证明其对维持染色质动态平衡的关键作用。

4. **文献名称**:IWS1缺失导致小鼠胚胎发育异常的机制

**作者**:Itoh M. et al.

**摘要**:基于IWS1抗体的组织免疫组化分析,发现IWS1敲除小鼠胚胎中神经管闭合缺陷,证实其通过调控Sox2等发育相关基因影响胚胎形态发生。

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注:以上文献为模拟内容,实际研究中建议通过PubMed或Web of Science查询真实文献。

背景信息

The IWS1 (Interacts with Spt6) antibody is a research tool used to study the IWS1 protein, a key regulator of transcriptional elongation and chromatin modification. IWS1 interacts with the transcription elongation factor Spt6 and RNA polymerase II, facilitating the recruitment of histone methyltransferases like SetD2 to promote H3K36me3 deposition, a mark associated with active transcription. This protein also plays a role in coupling transcription with mRNA export and surveillance. Dysregulation of IWS1 has been implicated in cancers, including leukemia and solid tumors, due to its influence on oncogenic gene expression programs. The antibody is commonly employed in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate IWS1's expression, localization, and interactions in cellular models. Studies using IWS1 antibodies have highlighted its phosphorylation-dependent regulation, particularly during cellular stress responses. Its role in epigenetic regulation and disease pathways makes it a focus in cancer biology and transcriptional research.

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