| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/10-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | hWNT5A |
| WB Predicted band size | 42 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human WNT5A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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1. **"WNT5A signaling contributes to β-catenin-independent cancer cell migration and invasion"**
- Author: Katoh, M. et al.
- 摘要：研究通过免疫组化和Western blot分析，发现WNT5A抗体检测显示其在黑色素瘤等肿瘤中高表达，激活非经典Wnt通路，促进肿瘤细胞迁移和侵袭。
2. **"WNT5A regulates epithelial differentiation in the developing lung via autocrine signaling"**
- Author: Li, C. et al.
- 摘要：利用WNT5A抗体进行组织染色和功能实验，揭示WNT5A通过调控细胞极性相关蛋白，影响肺发育过程中上皮细胞的分化和形态发生。
3. **"A monoclonal antibody against Wnt-5a inhibits postischemic angiogenesis"**
- Author: Blumenthal, A. et al.
- 摘要：开发了一种特异性WNT5A单克隆抗体，证明其可阻断WNT5A与受体结合，抑制缺血后血管生成，为治疗血管相关疾病提供潜在策略。
4. **"WNT5A induces inflammatory cytokine expression in human chondrocytes through a FZD5-dependent pathway"**
- Author: Ge, X. et al.
- 摘要：通过免疫沉淀和抗体阻断实验，发现WNT5A通过FZD5受体激活NF-κB通路，促进软骨细胞中IL-6和TNF-α的表达，参与骨关节炎炎症反应。
(注：上述文献为示例，实际引用时需核实具体作者及发表信息。)
The WNT5A antibody is a crucial tool for studying the WNT5A protein, a member of the Wnt family of secreted glycoproteins. WNT5A plays a pivotal role in non-canonical Wnt signaling pathways, such as planar cell polarity and Wnt/Ca²+ signaling, which regulate embryonic development, cell migration, polarity, and tissue homeostasis. Dysregulation of WNT5A is implicated in diverse pathologies, including cancer (e.g., melanoma, breast, and lung cancers), inflammatory diseases, and developmental disorders. Its dual role as a tumor promoter or suppressor, depending on cellular context, makes it a focus of oncology research.
WNT5A antibodies are widely used in techniques like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression, localization, and post-translational modifications. These antibodies target specific epitopes, with monoclonal antibodies (e.g., clones 3D2. 5F10-F8) offering high specificity, while polyclonal variants may detect multiple isoforms. Validation often involves recombinant protein reactivity or knockout cell line controls to ensure specificity, given WNT5A’s homology with other Wnt family members.
Research applications span cancer biology, developmental studies, and stem cell regulation. Challenges include cross-reactivity and variability in tissue-specific isoforms. Commercially available antibodies from suppliers like Abcam, Cell Signaling, and R&D Systems are optimized for distinct experimental workflows, emphasizing the need for context-dependent validation.
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