首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人内皮唾酸蛋白(CD248)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD248含量。CD248是一种在多种肿瘤和纤维化组织中高表达的跨膜糖蛋白。其背景上，与肿瘤血管生成、间质细胞增殖等密切相关。研究机制方面，它参与调节细胞间信号传导，促进肿瘤微环境形成，目前是肿瘤治疗和纤维化疾病研究的潜在重要靶点。试剂盒检测范围为0.312 ng/mL-20 ng/mL，本产品为研究CD248在实体瘤血管新生机制、炎症微环境动态变化、纤维化疾病进展等方向提供标准化工具，适用于细胞生物学实验、疾病模型动物研究及生物标志物探索等科研场景，助力靶向治疗基础研究与病理机制解析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CD164 sialomucin like 1 ELISA Kit; CD164L1 ELISA Kit; CD248 ELISA Kit; CD248 antigen endosialin ELISA Kit; CD248 molecule endosialin ELISA Kit; CD248_HUMAN ELISA Kit; Endosialin ELISA Kit; TEM 1 ELISA Kit; TEM1 ELISA Kit; Tumor endothelial marker 1 ELISA Kit
缩写：CD248
Uniprot No.：Q9HCU0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD248 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of human CD248 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 80-98 | ||||
| EDTA plasma (n=4) | 96 | 91-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected201.9091.8521.8811.741101.6961.7061.7011.56151.4441.4111.4281.2882.50.9520.9930.9730.8331.250.5830.5490.5660.4260.6250.3820.3950.3890.2490.3120.2180.2010.2100.07000.1420.1370.140 | ||||||
货期：3-5 working days
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