首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人CD226抗原(CD266)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CD226含量。CD226是一种糖蛋白，属于Ig超家族，主要表达于NK细胞、T细胞、单核细胞和血小板表面。它通过介导血小板和巨核细胞与血管内皮细胞的粘附，在巨核细胞成熟中发挥作用。CD226的研究涉及其在免疫细胞中的表达、细胞粘附、淋巴细胞信号传递、细胞毒作用以及与自身免疫疾病、感染性疾病和肿瘤的相关性。试剂盒检测范围为0.9 ng/mL-60 ng/mL，适用于基础科研领域，可帮助研究者探索CD226在免疫相关疾病模型中的表达变化、评估药物对免疫调节通路的影响，或分析不同组织微环境中CD226的生物学功能，为免疫学机制研究提供实验支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：adhesion glycoprotein ELISA Kit; CD226 ELISA Kit; CD226 antigen ELISA Kit; CD226 molecule ELISA Kit; CD226_HUMAN ELISA Kit; DNAM 1 ELISA Kit; DNAM-1 ELISA Kit; DNAM1 ELISA Kit; DNAX accessory molecule 1 ELISA Kit; platelet and T cell activation antigen 1 ELISA Kit; PTA1 ELISA Kit; T lineage specific activation antigen 1 antigen ELISA Kit; TLiSA1 ELISA Kit
缩写：CD226
Uniprot No.：Q15762
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.22 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CD226 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 85-91 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 87-93 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 90-96 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 84-92 | |||||
回收率：
| The recovery of human CD226 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-105 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.8012.8672.8342.732301.8661.8321.8491.747151.2161.2641.2401.1387.50.7990.7540.7770.6753.750.3630.3670.3650.2631.80.2560.2510.2540.1520.90.1700.1730.1720.07000.1010.1030.102 | ||||||
货期：3-5 working days
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