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产品描述:人天然杀伤细胞受体2B4(CD244)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CD244含量。CD244 是一种细胞表面受体。其背景在于它参与免疫细胞的调节等生理过程。研究机制上,它能与配体结合,影响免疫细胞活化、增殖和功能等,在免疫相关疾病如肿瘤免疫、感染免疫等研究中受到关注,有望为治疗提供新靶点。试剂盒检测范围为0.625 ng/mL-40 ng/mL,适用于探索CD244在NK细胞功能调控、病毒感染病理机制、肿瘤免疫微环境调控等领域的生物学作用,例如通过检测不同疾病模型中组织或血液样本的CD244动态变化,研究其与免疫细胞活化、免疫逃逸等过程的关联性。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:2B4 ELISA Kit; C9.1 ELISA Kit; CD244 ELISA Kit; CD244 antigen ELISA Kit; CD244 molecule ELISA Kit; CD244 molecule natural killer cell receptor 2B4 ELISA Kit; CD244 natural killer cell receptor 2B4 ELISA Kit; CD244_HUMAN ELISA Kit; F730046O15Rik ELISA Kit; h2B4 ELISA Kit; Ly90 ELISA Kit; NAIL ELISA Kit; Natural killer cell activation-inducing ligand ELISA Kit; Natural killer cell receptor 2B4 ELISA Kit; NK cell activation inducing ligand ELISA Kit; NK cell activation inducing ligand NAIL ELISA Kit; NK cell activation-inducing ligand ELISA Kit; NK cell type I receptor protein 2B4 ELISA Kit; NKR2B4 ELISA Kit; Nmrk ELISA Kit; Non-MHC restricted killing associated ELISA Kit; OTTHUMP00000027884 ELISA Kit; p38 ELISA Kit; signaling lymphocytic activation molecule 4 ELISA Kit; SLAM family, member 4 ELISA Kit; SLAMF4 ELISA Kit
缩写:CD244
Uniprot No.:Q9BZW8
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates, cell lysates
检测范围:0.625 ng/mL-40 ng/mL
灵敏度:0.156 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Immunology
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human CD244 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 91 | |||||
Range % | 88-96 | ||||||
1:2 | Average % | 102 | |||||
Range % | 98-106 | ||||||
1:4 | Average % | 99 | |||||
Range % | 95-103 | ||||||
1:8 | Average % | 89 | |||||
Range % | 85-93 |
回收率:
The recovery of human CD244 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 104 | 100-108 | |||||
EDTA plasma (n=4) | 97 | 93-100 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/mlOD1OD2AverageCorrected402.1732.2002.1872.049201.6041.7001.6521.514101.1181.2041.1611.02350.7650.7500.7580.6202.50.5030.4980.5010.3631.250.3350.3410.3380.2000.6250.2270.2310.2290.09100.1390.1360.138 |
货期:3-5 working days
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