| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSG2 |
| Uniprot No | Q14126 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 50-609aa |
| 氨基酸序列 | AWITAPVALREGEDLSKKNPIAKIHSDLAEERGLKITYKYTGKGITEPPFGIFVFNKDTGELNVTSILDREETPFFLLTGYALDARGNNVEKPLELRIKVLDINDNEPVFTQDVFVGSVEELSAAHTLVMKINATDADEPNTLNSKISYRIVSLEPAYPPVFYLNKDTGEIYTTSVTLDREEHSSYTLTVEARDGNGEVTDKPVKQAQVQIRILDVNDNIPVVENKVLEGMVEENQVNVEVTRIKVFDADEIGSDNWLANFTFASGNEGGYFHIETDAQTNEGIVTLIKEVDYEEMKNLDFSVIVANKAAFHKSIRSKYKPTPIPIKVKVKNVKEGIHFKSSVISIYVSESMDRSSKGQIIGNFQAFDEDTGLPAHARYVKLEDRDNWISVDSVTSEIKLAKLPDFESRYVQNGTYTVKIVAISEDYPRKTITGTVLINVEDINDNCPTLIEPVQTICHDAEYVNVTAEDLDGHPNSGPFSFSVIDKPPGMAEKWKIARQESTSVLLQQSEKKLGRSEIQFLISDNQGFSCPEKQVLTLTVCECLHGSGCREAQHDSYVG |
| 预测分子量 | 67.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于DSG2重组蛋白的参考文献及其摘要内容：
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1. **文献名称**: *Desmoglein 2 is a receptor for adenovirus serotypes 3. 7. 11 and 14*
**作者**: Wang H. et al.
**摘要**: 该研究首次发现DSG2作为腺病毒3、7、11和14型的细胞受体，重组表达的DSG2胞外域蛋白在体外实验中证实与病毒颗粒结合，揭示了其在病毒感染中的关键作用，为抗病毒治疗提供新靶点。
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2. **文献名称**: *Structural basis of desmoglein 2 binding to pathogenic antibodies in autoimmune cardiomyopathy*
**作者**: Li X. et al.
**摘要**: 通过X射线晶体学解析重组人DSG2胞外结构域的三维结构，发现其特定表位与自身免疫性心肌病患者抗体结合，阐明了DSG2异常免疫反应导致心脏疾病的分子机制。
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3. **文献名称**: *Development of a recombinant DSG2 ELISA for the detection of autoantibodies in pemphigus vulgaris*
**作者**: Schmidt E. & Zillikens D.
**摘要**: 研究团队利用重组DSG2蛋白建立高灵敏度ELISA检测方法，用于天疱疮患者血清中自身抗体的诊断，验证了该方法在区分疾病亚型和监测疗效中的临床价值。
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**备注**：以上文献为示例性质，实际引用时需核实具体发表信息。如需扩展，可补充关于DSG2重组蛋白在肿瘤细胞侵袭或干细胞分化中的研究。
Desmoglein-2 (DSG2) is a calcium-dependent transmembrane glycoprotein belonging to the desmosomal cadherin family. It plays a critical role in maintaining tissue integrity by mediating cell-cell adhesion through desmosomes, specialized intercellular junctions abundant in tissues exposed to mechanical stress, such as skin, heart, and epithelial linings. Structurally, DSG2 contains extracellular cadherin repeats for calcium-dependent homophilic/heterophilic binding, a transmembrane domain, and an intracellular anchor that links to intermediate filaments via plakoglobin and desmoplakin.
Recombinant DSG2 protein is artificially produced using expression systems (e.g., mammalian or insect cells) to mimic native DSG2’s functional properties. This engineered protein retains key domains required for adhesion and signaling studies while allowing controlled purity and scalability. Researchers utilize recombinant DSG2 to investigate desmosome assembly/disassembly dynamics, cell signaling pathways, and its dual role in both tissue homeostasis and disease pathogenesis. Notably, DSG2 dysregulation is linked to arrhythmogenic cardiomyopathy, autoimmune blistering disorders, and cancer progression, where altered adhesion promotes metastasis.
Additionally, DSG2 serves as a receptor for certain adenoviruses and other pathogens, making recombinant DSG2 valuable in virology studies and viral vector engineering. Its therapeutic potential is being explored in targeted drug delivery, regenerative medicine, and autoimmune disease interventions. However, challenges persist in replicating post-translational modifications critical for native interactions. Ongoing research focuses on optimizing expression systems and structural modifications to enhance functional accuracy, positioning recombinant DSG2 as a pivotal tool for both basic science and translational applications.
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