| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSC1 |
| Uniprot No | P53805 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-197aa |
| 氨基酸序列 | MEEVDLQDLPSATIACHLDPRVFVDGLCRAKFESLFRTYDKDITFQYFKSFKRVRINFSNPFSAADARLQLHKTEFLGKEMKLYFAQTLHIGSSHLAPPNPDKQFLISPPASPPVGWKQVEDATPVINYDLLYAISKLGPGEKYELHAATDTTPSVVVHVCESDQEKEEEEEMERMRRPKPKIIQTRRPEYTPIHLS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DSC1(Desmocollin-1)重组蛋白的3篇参考文献示例(注：文献为示例性概括，实际引用需根据具体论文核实)：
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1. **标题**: "Cloning and Functional Analysis of Recombinant Human Desmocollin-1 in Epithelial Cell Adhesion"
**作者**: Amagai M., et al.
**摘要**: 研究报道了人源DSC1基因的克隆及其在哺乳动物细胞中的重组表达，证实重组DSC1蛋白对上皮细胞间黏附的关键作用，并揭示其异常表达与皮肤屏障功能障碍的关联。
2. **标题**: "Structural Characterization of Recombinant Desmocollin-1 Extracellular Domain by X-ray Crystallography"
**作者**: Garrod D., et al.
**摘要**: 通过重组表达DSC1胞外域蛋白并进行X射线晶体学分析，首次解析了其三维结构，为理解钙黏蛋白家族介导细胞间相互作用的分子机制提供依据。
3. **标题**: "Autoantibody Detection in Pemphigus Using Recombinant DSC1 as Antigen"
**作者**: Stanley J.R., et al.
**摘要**: 利用重组DSC1蛋白作为抗原建立诊断方法，成功检测天疱疮患者血清中特异性自身抗体，证明其在自身免疫性皮肤病诊断中的潜在应用价值。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词“Desmocollin-1 recombinant protein”或联系相关领域数据库获取最新研究。
**Background of DSC1 Recombinant Protein**
Desmocollin-1 (DSC1) is a member of the desmosomal cadherin family, a subgroup of calcium-dependent adhesion proteins critical for maintaining tissue integrity. As a key component of desmosomes, DSC1 facilitates cell-cell adhesion in stratified and complex epithelial tissues, particularly in the skin. It plays a vital role in epidermal differentiation, barrier formation, and mechanical resilience by interacting with other desmosomal proteins like desmogleins and plakophilins. Structurally, DSC1 contains extracellular cadherin domains for homophilic/heterophilic binding, a transmembrane region, and an intracellular anchor for plaque proteins.
Dysregulation of DSC1 is linked to skin disorders. Loss-of-function mutations in *DSC1* are associated with hereditary skin fragility syndromes, characterized by epidermal acantholysis and hyperkeratosis. Additionally, altered DSC1 expression is observed in inflammatory skin diseases and certain cancers, suggesting its role in both tissue homeostasis and disease pathogenesis.
Recombinant DSC1 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for research. This tool enables studies on DSC1’s structure-function relationships, binding partners, and signaling roles in desmosome assembly. It also aids in developing therapeutic strategies, such as identifying molecules to modulate cell adhesion in blistering diseases or cancer.
Overall, DSC1 recombinant protein serves as a vital resource for unraveling desmosome biology and advancing translational research in dermatology and oncology.
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