| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSC2 |
| Uniprot No | Q02487 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-901aa |
| 氨基酸序列 | MEAARPSGSWNGALCRLLLLTLAILIFASDACKNVTLHVPSKLDAEKLVGRVNLKECFTAANLIHSSDPDFQILEDGSVYTTNTILLSSEKRSFTILLSNTENQEKKKIFVFLEHQTKVLKKRHTKEKVLRRAKRRWAPIPCSMLENSLGPFPLFLQQVQSDTAQNYTIYYSIRGPGVDQEPRNLFYVERDTGNLYCTRPVDREQYESFEIIAFATTPDGYTPELPLPLIIKIEDENDNYPIFTEETYTFTIFENCRVGTTVGQVCATDKDEPDTMHTRLKYSIIGQVPPSPTLFSMHPTTGVITTTSSQLDRELIDKYQLKIKVQDMDGQYFGLQTTSTCIINIDDVNDHLPTFTRTSYVTSVEENTVDVEILRVTVEDKDLVNTANWRANYTILKGNENGNFKIVTDAKTNEGVLCVVKPLNYEEKQQMILQIGVVNEAPFSREASPRSAMSTATVTVNVEDQDEGPECNPPIQTVRMKENAEVGTTSNGYKAYDPETRSSSGIRYKKLTDPTGWVTIDENTGSIKVFRSLDREAETIKNGIYNITVLASDQGGRTCTGTLGIILQDVNDNSPFIPKKTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWRLKAINDTAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTHRVDPRIGGGGVQLGKWAILAILLGIALLFCILFTLVCGASGTSKQPKVIPDDLAQQNLIVSNTEAPGDDKVYSANGFTTQTVGASAQGVCGTVGSGIKNGGQETIEMVKGGHQTSESCRGAGHHHTLDSCRGGHTEVDNCRYTYSEWHSFTQPRLGEKVYLCNQDENHKHAQDYVLTYNYEGRGSVAGSVGCCSERQEEDGLEFLDNLEPKFRTLAEACMKR |
| 预测分子量 | 99,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DSC2重组蛋白的模拟参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Recombinant expression and functional characterization of human Desmocollin 2 (DSC2) in epithelial cell adhesion"*
**作者**: Smith A, et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人DSC2蛋白，通过体外细胞粘附实验证明DSC2在维持上皮细胞间连接中起关键作用，并发现其功能依赖钙离子结合结构域。
2. **文献名称**: *"Structural insights into DSC2-mediated desmosome assembly via recombinant protein crystallization"*
**作者**: Lee C, et al.
**摘要**: 利用重组DSC2蛋白进行X射线晶体学分析，首次解析了DSC2胞外结构域的三维结构，揭示了其介导细胞间相互作用的分子机制，为遗传性皮肤病的治疗提供新靶点。
3. **文献名称**: *"DSC2 mutations and recombinant protein rescue in arrhythmogenic cardiomyopathy models"*
**作者**: Tanaka K, et al.
**摘要**: 构建携带DSC2基因突变的细胞模型，通过外源性重组DSC2蛋白恢复心肌细胞间连接功能，证实DSC2缺陷是致心律失常性心肌病的潜在病因之一。
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**注意**：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science或Google Scholar检索关键词“DSC2 recombinant protein”“Desmocollin 2 expression”等获取真实文献。
Desmocollin-2 (DSC2) is a member of the desmocollin subfamily of desmosomal cadherins, which are critical components of desmosomes—specialized intercellular junctions that mediate strong cell-cell adhesion and maintain tissue integrity. As a calcium-dependent transmembrane glycoprotein, DSC2 plays a vital role in anchoring intermediate filaments to the plasma membrane, particularly in tissues subjected to mechanical stress, such as cardiac muscle and epithelial layers. Dysregulation or mutations in DSC2 have been linked to arrhythmogenic cardiomyopathy (ACM), a heritable heart disorder characterized by fibrofatty myocardial replacement and arrhythmias, as well as skin blistering disorders like pemphigus.
Recombinant DSC2 protein is engineered using molecular cloning techniques to express and purify the protein in vitro, typically in systems like mammalian cells, insect cells, or bacteria. This allows researchers to study its structural and functional properties, including its role in desmosome assembly, cell adhesion dynamics, and interactions with other desmosomal components (e.g., desmogleins, plakoglobin). Recombinant DSC2 is often utilized to investigate pathogenic mechanisms of DSC2-associated diseases, develop diagnostic antibodies, or screen therapeutic compounds targeting desmosomal dysfunction. Its extracellular domain, which mediates homophilic and heterophilic binding, is a particular focus for structural studies and drug design. By enabling precise manipulation and analysis, recombinant DSC2 serves as a key tool in advancing both basic research and clinical applications related to cell adhesion disorders.
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