| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSG1 |
| Uniprot No | Q02413 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1049aa |
| 氨基酸序列 | MDWSFFRVVAMLFIFLVVVEVNSEFRIQVRDYNTKNGTIKWHSIRRQKRE WIKFAAACREGEDNSKRNPIAKIHSDCAANQQVTYRISGVGIDQPPYGIF VINQKTGEINITSIVDREVTPFFIIYCRALNSMGQDLERPLELRVRVLDI NDNPPVFSMATFAGQIEENSNANTLVMILNATDADEPNNLNSKIAFKIIR QEPSDSPMFIINRNTGEIRTMNNFLDREQYGQYALAVRGSDRDGGADGMS AECECNIKILDVNDNIPYMEQSSYTIEIQENTLNSNLLEIRVIDLDEEFS ANWMAVIFFISGNEGNWFEIEMNERTNVGILKVVKPLDYEAMQSLQLSIG VRNKAEFHHSIMSQYKLKASAISVTVLNVIEGPVFRPGSKTYVVTGNMGS NDKVGDFVATDLDTGRPSTTVRYVMGNNPADLLAVDSRTGKLTLKNKVTK EQYNMLGGKYQGTILSIDDNLQRTCTGTININIQSFGNDDRTNTEPNTKI TTNTGRQESTSSTNYDTSTTSTDSSQVYSSEPGNGAKDLLSDNVHFGPAG IGLLIMGFLVLGLVPFLMICCDCGGAPRSAAGFEPVPECSDGAIHSWAVE GPQPEPRDITTVIPQIPPDNANIIECIDNSGVYTNEYGGREMQDLGGGER MTGFELTEGVKTSGMPEICQEYSGTLRRNSMRECREGGLNMNFMESYFCQ KAYAYADEDEGRPSNDCLLIYDIEGVGSPAGSVGCCSFIGEDLDDSFLDT LGPKFKKLADISLGKESYPDLDPSWPPQSTEPVCLPQETEPVVSGHPPIS PHFGTTTVISESTYPSGPGVLHPKPILDPLGYGNVTVTESYTTSDTLKPS VHVHDNRPASNVVVTERVVGPISGADLHGMLEMPDLRDGSNVIVTERVIA PSSSLPTSLTIHHPRESSNVVVTERVIQPTSGMIGSLSMHPELANAHNVI VTERVVSGAGVTGISGTTGISGGIGSSGLVGTSMGAGSGALSGAGISGGG IGLSSLGGTASIGHMRSSSDHHFNQTIGSASPSTARSRITKYSTVQYSK |
| 预测分子量 | 142 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DSG1重组蛋白的模拟参考文献示例(注：以下内容为假设性文献，仅供参考格式和内容方向)：
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1. **文献名称**：*Recombinant DSG1 Expression and Its Role in Pemphigus Autoantibody Binding*
**作者**：Smith JL, et al.
**摘要**：本研究利用哺乳动物表达系统成功制备了重组DSG1胞外域蛋白，并证明其在体外可特异性结合天疱疮患者血清中的自身抗体，为疾病诊断提供了高灵敏度抗原工具。
2. **文献名称**：*Structural Analysis of DSG1 Ectodomain Reveals Pathogenic Epitopes*
**作者**：Chen R, et al.
**摘要**：通过X射线晶体学解析了重组DSG1蛋白的胞外结构域三维结构，鉴定出与自身免疫性疾病相关的关键抗原表位，为靶向药物设计奠定基础。
3. **文献名称**：*DSG1 Recombinant Protein Modulates Keratinocyte Adhesion in Vitro*
**作者**：Garcia M, et al.
**摘要**：研究发现，重组DSG1蛋白可竞争性抑制角质形成细胞的细胞间黏附，揭示了DSG1在表皮屏障功能中的动态调节机制及其在皮肤病理中的潜在作用。
4. **文献名称**：*Development of a DSG1-Based ELISA for Pemphigus Foliaceus Diagnosis*
**作者**：Tanaka K, et al.
**摘要**：基于重组DSG1蛋白开发的新型ELISA检测方法，显著提高了落叶型天疱疮的血清学诊断特异性，临床验证显示与传统免疫荧光法高度一致。
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**注意**：以上文献为示例性质，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表的论文。
Desmoglein 1 (DSG1) is a calcium-dependent transmembrane glycoprotein belonging to the desmosomal cadherin family, primarily expressed in stratified squamous epithelia, such as the skin and mucous membranes. It plays a critical role in maintaining cell-cell adhesion through desmosomes, specialized intercellular junctions that provide mechanical stability to tissues under stress. Structurally, DSG1 contains extracellular cadherin repeats for homophilic binding, a transmembrane domain, and an intracellular plakin-binding region that links to the keratin cytoskeleton. Dysregulation of DSG1 is implicated in autoimmune and genetic disorders. Autoantibodies targeting DSG1 disrupt cell adhesion in pemphigus foliaceus, causing blistering skin lesions, while mutations in the DSG1 gene are associated with severe dermatoses like SAM syndrome.
Recombinant DSG1 protein, produced via expression systems (e.g., mammalian or insect cells), retains key functional domains to mimic native DSG1’s structure and activity. It serves as a vital tool for studying desmosome dynamics, autoimmune pathogenesis, and genetic variants. Researchers use it in antibody neutralization assays, epitope mapping, and drug screening for pemphigus therapies. Additionally, recombinant DSG1 aids in developing diagnostic kits to detect pathogenic autoantibodies. Its standardized production ensures consistent quality, enabling mechanistic insights into epithelial integrity and translational applications in dermatology and immunology.
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