| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSC3 |
| Uniprot No | Q14574 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-896aa |
| 氨基酸序列 | MAAAGPRRSVRGAVCLHLLLTLVIFSRAGEACKKVILNVPSKLEADKIIGRVNLEECFRSADLIRSSDPDFRVLNDGSVYTARAVALSDKKRSFTIWLSDKRKQTQKEVTVLLEHQKKVSKTRHTRETVLRRAKRRWAPIPCSMQENSLGPFPLFLQQVESDAAQNYTVFYSISGRGVDKEPLNLFYIERDTGNLFCTRPVDREEYDVFDLIAYASTADGYSADLPLPLPIRVEDENDNHPVFTEAIYNFEVLESSRPGTTVGVVCATDRDEPDTMHTRLKYSILQQTPRSPGLFSVHPSTGVITTVSHYLDREVVDKYSLIMKVQDMDGQFFGLIGTSTCIITVTDSNDNAPTFRQNAYEAFVEENAFNVEILRIPIEDKDLINTANWRVNFTILKGNENGHFKISTDKETNEGVLSVVKPLNYEENRQVNLEIGVNNEAPFARDIPRVTALNRALVTVHVRDLDEGPECTPAAQYVRIKENLAVGSKINGYKAYDPENRNGNGLRYKKLHDPKGWITIDEISGSIITSKILDREVETPKNELYNITVLAIDKDDRSCTGTLAVNIEDVNDNPPEILQEYVVICKPKMGYTDILAVDPDEPVHGAPFYFSLPNTSPEISRLWSLTKVNDTAARLSYQKNAGFQEYTIPITVKDRAGQAATKLLRVNLCECTHPTQCRATSRSTGVILGKWAILAILLGIALLFSVLLTLVCGVFGATKGKRFPEDLAQQNLIISNTEAPGDDRVCSANGFMTQTTNNSSQGFCGTMGSGMKNGGQETIEMMKGGNQTLESCRGAGHHHTLDSCRGGHTEVDNCRYTYSEWHSFTQPRLGEKLHRCNQNEDRMPSQDYVLTYNYEGRGSPAGSVGCCSEKQEEDGLDFLNNLEPKFITLAEACTKR |
| 预测分子量 | 99,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Recombinant DSC3 regulates epithelial-mesenchymal transition in oral squamous cell carcinoma"**
*Author: Li et al.*
**摘要**: 研究探讨了重组DSC3蛋白通过调控E-cadherin和N-cadherin表达抑制口腔鳞癌细胞EMT进程，揭示其通过Wnt/β-catenin通路抑制肿瘤转移的机制。
2. **"Expression and purification of functional human DSC3 extracellular domain in HEK293 cells"**
*Author: Müller et al.*
**摘要**: 报道了利用HEK293细胞系统高效表达具有生物学活性的DSC3胞外域重组蛋白，并通过免疫共沉淀验证其与桥粒蛋白DSG3的相互作用，为结构研究提供工具。
3. **"DSC3 recombinant protein ameliorates skin barrier defects in psoriasis models"**
*Author: García et al.*
**摘要**: 在银屑病小鼠模型中，局部应用重组DSC3蛋白恢复了表皮桥粒完整性，降低IL-17/IL-23炎症因子水平，证实其修复皮肤屏障的潜力。
4. **"DSC3 loss promotes pancreatic cancer metastasis via desmosome disruption"**
*Author: Wang et al.*
**摘要**: 通过重组DSC3回补实验证明，DSC3缺失导致胰腺癌细胞桥粒结构破坏，激活FAK/Src信号通路，促进侵袭转移，提示其作为转移抑制因子的功能。
(注：以上文献为示例性虚构内容，实际研究需通过PubMed/Google Scholar检索关键词如"DSC3 recombinant protein")
Desmocollin-3 (DSC3) is a member of the desmocollin family, a subset of calcium-dependent transmembrane glycoproteins within the cadherin superfamily. It serves as a critical component of desmosomes, specialized intercellular junctions that mediate cell-cell adhesion and maintain tissue integrity, particularly in mechanically stressed epithelia such as the skin and heart. DSC3 interacts with desmogleins and intracellular plaque proteins (e.g., plakoglobin, desmoplakin) to anchor intermediate filaments, ensuring structural cohesion and signal transduction across tissues.
Dysregulation of DSC3 is implicated in pathological conditions. Loss-of-function mutations or reduced expression disrupts desmosome assembly, contributing to skin blistering disorders like pemphigus vulgaris or arrhythmogenic cardiomyopathy. Conversely, DSC3 overexpression or aberrant localization has been observed in cancers, including squamous cell carcinomas, where it may influence tumor progression, metastasis, and therapeutic resistance through EMT (epithelial-mesenchymal transition) modulation.
Recombinant DSC3 protein, produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells), enables mechanistic studies of its role in cell adhesion, signaling, and disease. Engineered variants (e.g., ectodomain fragments, tagged proteins) are used to investigate binding partners, autoantibody interactions in autoimmune disorders, or as antigens for diagnostic assays. Recent studies also explore DSC3's potential as a therapeutic target, leveraging recombinant proteins to develop blocking antibodies or adhesion-modulating peptides. Its dual role in tissue homeostasis and disease underscores its relevance in both basic research and translational applications.
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