| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUOX2 |
| Uniprot No | Q9NRD8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1548aa |
| 氨基酸序列 | MLRARPEALMLLGALLTGSLGPSGNQDALSLPWEVQRYDGWFNNLRHHERGAVGCRLQRRVPANYADGVYQALEEPQLPNPRRLSNAATRGIAGLPSLHNRTVLGVFFGYHVLSDVVSVETPGCPAEFLNIRIPPGDPVFDPDQRGDVVLPFQRSRWDPETGRSPSNPRDLANQVTGWLDGSAIYGSSHSWSDALRSFSGGQLASGPDPAFPRDSQNPLLMWAAPDPATGQNGPRGLYAFGAERGNREPFLQALGLLWFRYHNLWAQRLARQHPDWEDEELFQHARKRVIATYQNIAVYEWLPSFLQKTLPEYTGYRPFLDPSISPEFVVASEQFFSTMVPPGVYMRNASCHFRKVLNKGFQSSQALRVCNNYWIRENPNLNSTQEVNELLLGMASQISELEDNIVVEDLRDYWPGPGKFSRTDYVASSIQRGRDMGLPSYSQALLAFGLDIPRNWSDLNPNVDPQVLEATAALYNQDLSQLELLLGGLLESHGDPGPLFSAIVLDQFVRLRDGDRYWFENTRNGLFSKKEIEDIRNTTLRDVLVAVINIDPSALQPNVFVWHKGAPCPQPKQLTTDGLPQCAPLTVLDFFEGSSPGFAITIIALCCLPLVSLLLSGVVAYFRGREHKKLQKKLKESVKKEAAKDGVPAMEWPGPKERSSPIIIQLLSDRCLQVLNRHLTVLRVVQLQPLQQVNLILSNNRGCRTLLLKIPKEYDLVLLFSSEEERGAFVQQLWDFCVRWALGLHVAEMSEKELFRKAVTKQQRERILEIFFRHLFAQVLDINQADAGTLPLDSSQKVREALTCELSRAEFAESLGLKPQDMFVESMFSLADKDGNGYLSFREFLDILVVFMKGSPEDKSRLMFTMYDLDENGFLSKDEFFTMMRSFIEISNNCLSKAQLAEVVESMFRESGFQDKEELTWEDFHFMLRDHDSELRFTQLCVKGGGGGGNGIRDIFKQNISCRVSFITRTPGERSHPQGLGPPAPEAPELGGPGLKKRFGKKAAVPTPRLYTEALQEKMQRGFLAQKLQQYKRFVENYRRHIVCVAIFSAICVGVFADRAYYYGFASPPSDIAQTTLVGIILSRGTAASVSFMFSYILLTMCRNLITFLRETFLNRYVPFDAAVDFHRWIAMAAVVLAILHSAGHAVNVYIFSVSPLSLLACIFPNVFVNDGSKLPQKFYWWFFQTVPGMTGVLLLLVLAIMYVFASHHFRRRSFRGFWLTHHLYILLYALLIIHGSYALIQLPTFHIYFLVPAIIYGGDKLVSLSRKKVEISVVKAELLPSGVTYLQFQRPQGFEYKSGQWVRIACLALGTTEYHPFTLTSAPHEDTLSLHIRAVGPWTTRLREIYSSPKGNGCAGYPKLYLDGPFGEGHQEWHKFEVSVLVGGGIGVTPFASILKDLVFKSSLGSQMLCKKIYFIWVTRTQRQFEWLADIIQEVEENDHQDLVSVHIYVTQLAEKFDLRTTMLYICERHFQKVLNRSLFTGLRSITHFGRPPFEPFFNSLQEVHPQVRKIGVFSCGPPGMTKNVEKACQLVNRQDRAHFMHHYENF |
| 预测分子量 | 175,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DUOX2重组蛋白的3篇典型研究方向及相关文献示例(注：文献信息为示例性质，实际文献需通过学术数据库检索)：
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1. **文献名称**：*"Recombinant DUOX2 Expression in HEK293 Cells: Insights into Enzymatic Activation and Thyroid Pathogenesis"*
**作者**：Dupuy C, et al.
**摘要**：研究利用HEK293细胞系统重组表达人DUOX2蛋白，揭示了其依赖钙离子激活的过氧化氢生成机制，并发现特定突变(如R434Q)导致酶活性丧失，与先天性甲状腺功能减退症相关。
2. **文献名称**：*"Structural and Functional Analysis of DUOX2 Maturation and Trafficking in Thyroid Cells"*
**作者**：De Deken X, et al.
**摘要**：通过重组DUOX2蛋白在甲状腺细胞中的表达，阐明其在内质网中的成熟过程及伴侣蛋白DUOXA2的关键作用，证明二者共表达是维持酶活性的必要条件。
3. **文献名称**：*"DUOX2 Mutations Impair Reactive Oxygen Species Generation in Intestinal Organoids"*
**作者**：Grasberger H, et al.
**摘要**：利用重组DUOX2突变体构建肠道类器官模型，发现突变导致上皮细胞过氧化氢生成缺陷，影响黏液屏障功能，提示DUOX2在肠道先天免疫中的核心作用。
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**检索建议**：
- 使用PubMed或Google Scholar，关键词：**DUOX2 recombinant protein expression**、**DUOX2 mutations functional analysis**、**DUOX2 H2O2 generation**。
- 关注期刊：*Journal of Biological Chemistry*、*Thyroid*、*Antioxidants & Redox Signaling*。
如需具体文献全文链接或发表年份，可进一步提供检索指导。
**Background of DUOX2 Recombinant Protein**
DUOX2 (Dual Oxidase 2) is a member of the NADPH oxidase family, primarily expressed in epithelial tissues such as the thyroid, gastrointestinal tract, and respiratory system. It plays a critical role in generating hydrogen peroxide (H₂O₂), a key substrate for thyroid hormone biosynthesis via thyroid peroxidase. DUOX2 works in tandem with its maturation factor, DUOXA2. to form a functional enzyme complex on cell membranes. Mutations in the *DUOX2* gene are linked to congenital hypothyroidism and have been implicated in inflammatory conditions like inflammatory bowel disease (IBD) and chronic respiratory disorders.
Recombinant DUOX2 protein is engineered to study its enzymatic activity, structure-function relationships, and interactions with regulatory partners. Produced using mammalian or insect cell expression systems, the recombinant form often includes tags (e.g., His-tag) for purification and detection. Its applications span *in vitro* studies to dissect H₂O₂ production mechanisms, screen for enzyme inhibitors/activators, and model DUOX2-associated pathologies. Researchers also utilize it to develop therapeutic strategies targeting oxidative stress or autoimmune disorders linked to DUOX2 dysfunction.
Despite challenges in expressing this membrane-bound protein, advances in structural biology and recombinant technology continue to enhance its utility in both basic research and drug discovery. Understanding DUOX2’s role in health and disease remains pivotal for addressing thyroid disorders and inflammation-related conditions.
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