| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP5 |
| Uniprot No | Q16690 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-384aa |
| 氨基酸序列 | MKVTSLDGRQ LRKMLRKEAA ARCVVLDCRP YLAFAASNVR GSLNVNLNSV VLRRARGGAV SARYVLPDEA ARARLLQEGG GGVAAVVVLD QGSRHWQKLR EESAARVVLT SLLACLPAGP RVYFLKGGYE TFYSEYPECC VDVKPISQEK IESERALISQ CGKPVVNVSY RPAYDQGGPV EILPFLYLGS AYHASKCEFL ANLHITALLN VSRRTSEACA THLHYKWIPV EDSHTADISS HFQEAIDFID CVREKGGKVL VHCEAGISRS PTICMAYLMK TKQFRLKEAF DYIKQRRSMV SPNFGFMGQL LQYESEILPS TPNPQPPSCQ GEAAGSSLIG HLQTLSPDMQ GAYCTFPASV LAPVPTHSTV SELSRSPVAT ATSC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DUSP5重组蛋白的示例参考文献(内容为虚构，仅供格式参考)：
1. **"Recombinant DUSP5 Expression and Phosphatase Activity in MAPK Signaling"**
- **作者**: Smith A, et al.
- **摘要**: 研究报道了通过大肠杆菌系统高效表达并纯化重组DUSP5蛋白，验证其特异性去磷酸化ERK1/2的能力，揭示了其在负调控MAPK信号通路中的作用。
2. **"Structural Insights into DUSP5 Substrate Specificity by X-ray Crystallography"**
- **作者**: Chen L, et al.
- **摘要**: 通过X射线晶体学解析了重组DUSP5的三维结构，发现其N端结构域与ERK2的相互作用机制，为设计靶向DUSP5的小分子抑制剂提供结构基础。
3. **"DUSP5 Recombinant Protein Attenuates Tumor Growth in a Mouse Model"**
- **作者**: Wang Y, et al.
- **摘要**: 利用重组DUSP5蛋白进行体内实验，证明其过表达可抑制肿瘤微环境中ERK过度活化，显著延缓肺癌小鼠模型的肿瘤进展。
4. **"DUSP5 Regulates Inflammatory Responses via TLR4 Pathway Modulation"**
- **作者**: Kim H, et al.
- **摘要**: 研究通过体外重组DUSP5蛋白处理巨噬细胞，发现其通过负调控p38 MAPK磷酸化，降低炎症因子TNF-α和IL-6的分泌。
**提示**：实际文献需通过PubMed、Google Scholar等平台检索关键词(如"DUSP5 recombinant protein")。建议结合具体研究方向筛选近年高引论文或权威期刊文章。
DUSP5 (Dual Specificity Phosphatase 5), also known as mitogen-activated protein kinase phosphatase 3 (MKP-3), is a member of the dual-specificity phosphatase family that regulates mitogen-activated protein kinase (MAPK) signaling pathways. It specifically dephosphorylates and inactivates extracellular signal-regulated kinases 1 and 2 (ERK1/2), key mediators of cellular responses to growth factors, stress, and differentiation signals. Unlike cytoplasmic DUSPs, DUSP5 is predominantly nuclear, enabling localized control of ERK activity in the nucleus, which influences gene expression, cell cycle progression, and apoptosis. Its expression is tightly regulated by transcriptional and post-transcriptional mechanisms, often induced by ERK activation itself, forming a negative feedback loop to modulate signaling intensity and duration.
Recombinant DUSP5 protein is engineered for in vitro and in vivo studies to explore its regulatory mechanisms and therapeutic potential. Produced via bacterial or mammalian expression systems, it retains enzymatic activity to dephosphorylate ERK in experimental models. Researchers utilize it to investigate DUSP5’s role in diseases linked to dysregulated MAPK signaling, such as cancer, cardiovascular disorders, and inflammatory conditions. For example, DUSP5 downregulation has been observed in certain cancers, correlating with hyperactive ERK signaling and tumor progression. Conversely, its overexpression may suppress oncogenic phenotypes, highlighting its context-dependent tumor-suppressive functions. Recombinant DUSP5 also aids in drug discovery, serving as a target or tool to screen phosphatase modulators. Its structural domains, including the N-terminal kinase-binding motif and C-terminal catalytic domain, are critical for substrate recognition and enzyme activity, making them focal points for mechanistic and therapeutic studies. Overall, DUSP5 recombinant protein bridges molecular insights into MAPK pathway dynamics and translational applications in precision medicine.
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