| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP1 |
| Uniprot No | P28562 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-367aa |
| 氨基酸序列 | MVMEVGTLDA GGLRALLGER AAQCLLLDCR SFFAFNAGHI AGSVNVRFST IVRRRAKGAM GLEHIVPNAE LRGRLLAGAY HAVVLLDERS AALDGAKRDG TLALAAGALC REARAAQVFF LKGGYEAFSA SCPELCSKQS TPMGLSLPLS TSVPDSAESG CSSCSTPLYD QGGPVEILPF LYLGSAYHAS RKDMLDALGI TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA IDFIDSIKNA GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHST NSALSYLQSP ITTSPSC |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Characterization of recombinant human DUSP1/MKP-1 in the regulation of MAP kinase pathways"**
- **作者**: Franklin CC, et al.
- **摘要**: 研究报道了重组人源DUSP1蛋白的表达与纯化方法，并验证其通过去磷酸化ERK、JNK和p38 MAPK调控炎症反应的功能，为MAPK信号抑制机制提供依据。
2. **"DUSP1重组蛋白通过抑制JNK通路减轻心肌缺血再灌注损伤"**
- **作者**: 李明, 等.
- **摘要**: 利用原核系统表达重组DUSP1.发现其通过抑制JNK磷酸化减少心肌细胞凋亡，提示其在心血管疾病治疗中的潜在应用。
3. **"Structural and functional analysis of DUSP1 catalytic domain"**
- **作者**: Chen Y, et al.
- **摘要**: 通过晶体结构解析重组DUSP1催化域，揭示其底物结合特异性及活性位点突变对酶活的影响，为靶向药物设计提供结构基础。
4. **"Recombinant DUSP1 suppresses LPS-induced cytokine production in macrophages"**
- **作者**: Wang H, et al.
- **摘要**: 研究证明重组DUSP1蛋白可显著降低巨噬细胞中LPS诱导的TNF-α和IL-6分泌，机制涉及p38 MAPK通路负反馈调控。
(注：以上为模拟文献，实际引用请核实真实论文。)
**Background of DUSP1 Recombinant Protein**
DUSP1 (Dual Specificity Phosphatase 1), also known as MKP-1 (MAP Kinase Phosphatase-1), is a member of the dual-specificity phosphatase family that regulates mitogen-activated protein kinase (MAPK) signaling pathways. It functions as a critical negative feedback regulator by dephosphorylating and inactivating MAPKs, including ERK, JNK, and p38. which are involved in cellular responses to stress, inflammation, proliferation, and apoptosis. DUSP1 expression is tightly regulated at transcriptional and post-translational levels, often induced by stimuli such as growth factors, hormones, or cellular stressors.
The recombinant DUSP1 protein is produced using genetic engineering techniques, typically expressed in *E. coli* or mammalian systems, to study its structure, function, and interactions in controlled experimental settings. Structurally, DUSP1 contains an N-terminal catalytic domain responsible for phosphatase activity and a C-terminal regulatory domain that mediates substrate binding and subcellular localization.
Research on DUSP1 recombinant protein has highlighted its role in modulating immune responses, cancer progression, and neurodegenerative diseases. For example, DUSP1 deficiency or overexpression has been linked to dysregulated inflammation in sepsis, autoimmune disorders, and tumor survival. In cancer, DUSP1 exhibits context-dependent roles, acting as either a tumor suppressor by limiting oncogenic MAPK signaling or a promoter of chemoresistance.
Therapeutic targeting of DUSP1 is being explored, though challenges remain due to its tissue-specific effects and complex regulation. Recombinant DUSP1 serves as a valuable tool for elucidating MAPK pathway dynamics, screening drug candidates, and developing strategies to manipulate its activity in disease models. Ongoing studies focus on deciphering its structure-function relationships, post-translational modifications, and interplay with other signaling molecules to harness its potential in precision medicine.
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