| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DCX |
| Uniprot No | O43602 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-441aa |
| 氨基酸序列 | MKTLPLHSHCTEMQRLLPKLEMLTLGSSFCSLQGEFCQAMDSFTTVSHVGMCEETDASFNVFSPKFQFDRSHCQSLRFHQNMELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM |
| 预测分子量 | 65.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DCX(Doublecortin)重组蛋白的3篇代表性文献的模拟概括(内容基于领域内研究趋势，具体信息建议通过学术数据库核实)：
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1. **文献名称**：*Doublecortin, a Brain-Specific Gene Mutated in Human X-Linked Lissencephaly and Double Cortex Syndrome, Encodes a Putative Signaling Protein*
**作者**：Gleeson, J. G., et al.
**摘要**：该研究首次克隆了DCX基因，并发现其在人类X连锁无脑回畸形和双皮质综合征中的关键作用。作者通过重组蛋白表达验证了DCX与微管结合的活性，揭示了其在大脑皮层发育中调控神经元迁移的分子机制。
2. **文献名称**：*Phosphorylation of DCX by Cdk5 regulates its function in neuronal migration*
**作者**：Sapir, T., et al.
**摘要**：研究探讨了重组DCX蛋白的翻译后修饰机制，发现细胞周期蛋白依赖性激酶5(Cdk5)通过磷酸化DCX的特定丝氨酸残基，调控其与微管的结合能力，从而影响神经元迁移过程中的细胞骨架动态变化。
3. **文献名称**：*Recombinant DCX protein enhances neurogenesis in vitro and promotes axonal regeneration after spinal cord injury*
**作者**：Francis, F., et al.
**摘要**：利用重组DCX蛋白处理神经干细胞，发现其显著促进神经元分化并抑制胶质细胞生成。动物实验表明，局部递送重组DCX可改善脊髓损伤后的轴突再生，为神经修复提供了潜在治疗策略。
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**备注**：以上文献信息为模拟示例，实际研究需参考具体论文。建议通过PubMed或Web of Science检索关键词“Doublecortin recombinant protein”获取最新文献。
**Background of DCX Recombinant Protein**
Doublecortin (DCX) is a microtubule-associated protein predominantly expressed in migrating neurons during embryonic brain development. It plays a critical role in neuronal migration, cortical layering, and neurogenesis, particularly in the formation of the cerebral cortex and hippocampus. DCX mutations are linked to neurodevelopmental disorders such as X-linked lissencephaly and subcortical band heterotopia (double cortex syndrome), highlighting its importance in proper brain architecture.
The DCX gene encodes a 40 kDa protein containing two conserved tandem doublecortin (DC) domains that bind to microtubules, stabilizing and promoting their polymerization. This activity is essential for neuronal motility and the extension of axons and dendrites. Recombinant DCX protein is engineered in vitro using expression systems like *E. coli* or mammalian cell lines, enabling researchers to study its structure, interactions, and functional mechanisms in a controlled environment.
DCX recombinant protein is widely utilized in neuroscience research to investigate neurodevelopmental pathways, neuronal differentiation, and the molecular basis of brain malformations. It serves as a tool for in vitro assays, such as microtubule-binding studies, and as a biomarker for immature neurons in neurogenesis research. Additionally, it aids in drug discovery efforts targeting microtubule dynamics in neurological diseases.
Recent studies also explore DCX's role in adult neurogenesis and its potential implications in neurodegenerative disorders and brain repair. The availability of recombinant DCX has accelerated mechanistic insights into neuronal migration defects and opened avenues for therapeutic interventions in neurodevelopmental and degenerative conditions.
In summary, DCX recombinant protein is a vital resource for unraveling the complexities of brain development, neuronal plasticity, and associated pathologies. Its applications span basic research, diagnostic tools, and translational medicine.
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