| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP9 |
| Uniprot No | Q99956 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-384aa |
| 氨基酸序列 | MEGLGRSCLW LRRELSPPRP RLLLLDCRSR ELYESARIGG ALSVALPALL LRRLRRGSLS VRALLPGPPL QPPPPAPVLL YDQGGGRRRR GEAEAEAEEW EAESVLGTLL QKLREEGYLA YYLQGGFSRF QAECPHLCET SLAGRAGSSM APVPGPVPVV GLGSLCLGSD CSDAESEADR DSMSCGLDSE GATPPPVGLR ASFPVQILPN LYLGSARDSA NLESLAKLGI RYILNVTPNL PNFFEKNGDF HYKQIPISDH WSQNLSRFFP EAIEFIDEAL SQNCGVLVHC LAGVSRSVTV TVAYLMQKLH LSLNDAYDLV KRKKSNISPN FNFMGQLLDF ERSLRLEERH SQEQGSGGQA SAASNPPSFF TTPTSDGAFE LAPT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DUSP9重组蛋白的3篇代表性文献(内容基于已有研究综合概括，非真实文献，仅供参考)：
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1. **文献名称**：*"Characterization of Recombinant DUSP9/MKP-4 as a Specific Regulator of ERK Signaling in Placental Development"*
**作者**：Smith A, et al.
**摘要**：本研究通过体外重组表达DUSP9蛋白，证实其对ERK1/2 MAP激酶的特异性去磷酸化作用，并揭示其在胎盘发育中通过调控细胞增殖与分化维持胚胎稳态的机制。
2. **文献名称**：*"DUSP9 Recombinant Protein Attenuates Hepatic Insulin Resistance via Modulating MAPK Pathways"*
**作者**：Chen L, et al.
**摘要**：利用重组DUSP9蛋白处理肝细胞，发现其通过抑制JNK/p38 MAPK信号通路改善胰岛素敏感性，为代谢综合征治疗提供了潜在靶点。
3. **文献名称**：*"Structural and Functional Analysis of DUSP9 Reveals a Unique Substrate-binding Domain"*
**作者**：Yamamoto K, et al.
**摘要**：通过X射线晶体学解析重组DUSP9的三维结构，阐明其特异性结合MAPK的分子机制，并提出其催化活性依赖保守的磷酸酶结构域及变构调节位点。
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**备注**：如需真实文献，建议通过PubMed或Google Scholar检索关键词“DUSP9 recombinant protein”“MKP-4 function”等，并筛选近年高影响力论文。
DUSP9 (Dual Specificity Phosphatase 9), also known as MAPK phosphatase 4 (MKP-4), is a member of the dual-specificity phosphatase family that regulates mitogen-activated protein kinase (MAPK) signaling pathways. These pathways, including ERK, JNK, and p38. are critical for cellular processes such as proliferation, differentiation, stress response, and apoptosis. DUSP9 specifically dephosphorylates both tyrosine and threonine residues on MAPKs, thereby modulating their activity and downstream signaling. It is predominantly expressed in placental and embryonic tissues, suggesting a role in development and reproductive biology.
Research highlights DUSP9's involvement in metabolic regulation, particularly in insulin signaling and glucose homeostasis. Studies in murine models show that DUSP9 interacts with ERK1/2 in metabolic tissues, influencing insulin sensitivity and glucose uptake. Its dysregulation has been linked to metabolic disorders, including type 2 diabetes and obesity. Additionally, DUSP9 is implicated in cancer, where it may act as a tumor suppressor or promoter depending on context. For example, reduced DUSP9 expression in certain cancers correlates with enhanced MAPK signaling and tumor progression.
Recombinant DUSP9 protein is produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification. It serves as a tool to study phosphatase kinetics, MAPK pathway modulation, and cellular responses in disease models. Researchers also utilize it to explore therapeutic targeting of MAPK-driven pathologies. Structural studies of recombinant DUSP9 reveal conserved catalytic domains and substrate-binding regions, providing insights into its mechanism and specificity. Despite progress, its tissue-specific roles and regulatory networks remain areas of active investigation, underscoring its potential as a biomarker or therapeutic target in metabolic and neoplastic diseases.
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