| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ACTC1 |
| Uniprot No | P68032 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 3-377aa |
| 氨基酸序列 | DDEETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF |
| 预测分子量 | 47.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ACTC1重组蛋白的3篇参考文献示例(注：文献信息为模拟概括，实际文献需通过学术数据库验证)：
---
1. **文献名称**：*Expression and purification of recombinant human ACTC1 in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：研究报道了通过大肠杆菌表达系统成功克隆并纯化人源ACTC1重组蛋白，优化了表达条件以提高蛋白溶解度，并通过质谱和圆二色谱验证了其结构与天然蛋白的一致性。
2. **文献名称**：*Functional analysis of ACTC1 mutations using recombinant protein models*
**作者**：Chen L, et al.
**摘要**：利用重组ACTC1蛋白构建了与扩张型心肌病相关的突变体(如p.Glu101Lys)，通过体外肌动蛋白聚合实验，揭示了突变对细胞骨架动力学的影响。
3. **文献名称**：*ACTC1 recombinant protein interactions with cardiac myosin isoforms*
**作者**：Wang Y, et al.
**摘要**：通过表面等离子共振技术(SPR)分析了重组ACTC1与不同心肌肌球蛋白亚型的结合能力，为心肌收缩机制研究提供了分子层面证据。
---
**建议**：以上内容基于ACTC1研究的典型方向推测，具体文献请通过PubMed、Web of Science等平台以关键词“ACTC1 recombinant protein”检索获取最新数据。
**Background of ACTC1 Recombinant Protein**
ACTC1 (Alpha-Cardiac Actin 1) is a key structural protein predominantly expressed in cardiac muscle cells, where it plays a vital role in maintaining the contractile apparatus of the heart. As a member of the actin family, ACTC1 is essential for sarcomere organization, enabling force generation during myocardial contraction. Mutations in the *ACTC1* gene are linked to inherited cardiomyopathies, such as hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM), highlighting its critical role in cardiac function.
Recombinant ACTC1 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cell cultures) to express the purified protein for research and therapeutic applications. Its production typically involves cloning the human *ACTC1* gene into expression vectors, followed by protein extraction, purification (via affinity chromatography), and validation (e.g., SDS-PAGE, Western blot).
Studying recombinant ACTC1 allows researchers to investigate its structural and functional properties, including interactions with binding partners like myosin and tropomyosin. This protein is also utilized to model disease mechanisms caused by mutations, screen potential drugs targeting actin-related pathologies, and explore gene therapy strategies. Additionally, it serves as an antigen for antibody development in diagnostic assays.
The demand for high-purity ACTC1 recombinant protein continues to grow, driven by its significance in understanding cardiac biology and developing treatments for heart diseases. Ongoing research aims to refine production techniques and expand its applications in translational medicine.
×
