| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDHH |
| Uniprot No | P55290 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 139-693aa |
| 氨基酸序列 | SI VVSPILIPEN QRQPFPRDVG KVVDSDRPER SKFRLTGKGV DQEPKGIFRI NENTGSVSVT RTLDREVIAV YQLFVETTDV NGKTLEGPVP LEVIVIDQND NRPIFREGPY IGHVMEGSPT GTTVMRMTAF DADDPATDNA LLRYNIRQQT PDKPSPNMFY IDPEKGDIVT VVSPALLDRE TLENPKYELI IEAQDMAGLD VGLTGTATAT IMIDDKNDHS PKFTKKEFQA TVEEGAVGVI VNLTVEDKDD PTTGAWRAAY TIINGNPGQS FEIHTNPQTN EGMLSVVKPL DYEISAFHTL LIKVENEDPL VPDVSYGPSS TATVHITVLD VNEGPVFYPD PMMVTRQEDL SVGSVLLTVN ATDPDSLQHQ TIRYSVYKDP AGWLNINPIN GTVDTTAVLD RESPFVDNSV YTALFLAIDS GNPPATGTGT LLITLEDVND NAPFIYPTVA EVCDDAKNLS VVILGASDKD LHPNTDPFKF EIHKQAVPDK VWKISKINNT HALVSLLQNL NKANYNLPIM VTDSGKPPMT NITDLRVQVC SCRNSKVDCN AAG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于CDHH(假设为钙黏蛋白家族相关)重组蛋白的虚构参考文献示例，供参考：
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1. **标题**：Expression and Functional Analysis of Recombinant CDHH in Epithelial Cell Adhesion
**作者**：Zhang L, et al.
**摘要**：研究报道了人源CDHH重组蛋白在大肠杆菌中的可溶性表达及纯化方法，验证了其在体外促进上皮细胞间粘附的功能，并揭示了其钙离子依赖性相互作用机制。
2. **标题**：Structural Characterization of CDHH Recombinant Protein for Cancer Metastasis Studies
**作者**：Smith J, Patel R.
**摘要**：通过X射线晶体学解析了CDHH重组蛋白的胞外结构域三维构象，发现其结构异常与肿瘤细胞迁移能力增强相关，为靶向治疗提供了依据。
3. **标题**：CDHH Recombinant Protein-Based Screening Platform for Drug Discovery
**作者**：Kim S, et al.
**摘要**：开发了一种基于CDHH重组蛋白的高通量筛选模型，成功鉴定出可调节细胞-基质相互作用的先导化合物，应用于纤维化疾病药物研发。
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注：以上文献为示例性质，实际研究中请根据具体蛋白名称(如CDH1/CDH2等)和方向检索真实文献。
CDHH recombinant protein is a engineered protein construct designed for biomedical research and therapeutic applications. The term "CDHH" typically refers to a chimeric or hybrid protein combining functional domains from distinct proteins, often including cell adhesion molecules like cadherins (CDH) and other modular domains (denoted as "HH"). Cadherins are calcium-dependent transmembrane proteins critical for cell-cell adhesion, tissue morphogenesis, and cellular signaling. The classical cadherin family, such as E-cadherin (CDH1) and N-cadherin (CDH2), plays pivotal roles in maintaining tissue integrity and regulating epithelial-mesenchymal transitions.
Recombinant CDHH proteins are synthesized using genetic engineering techniques, where DNA sequences encoding specific cadherin extracellular domains are fused with heterologous functional motifs (e.g., immunoglobulin domains, growth factor receptors, or fluorescent tags) to enhance stability, solubility, or tracking capabilities. These proteins are commonly expressed in mammalian, insect, or bacterial expression systems, followed by purification via affinity chromatography.
In research, CDHH recombinant proteins serve as tools to study cell adhesion mechanisms, cancer metastasis, and developmental biology. They enable in vitro reconstitution of intercellular interactions, screening for cadherin-targeted therapeutics, or deciphering signaling pathways involving β-catenin and Wnt. Therapeutically, engineered cadherin variants are explored for tissue regeneration, anti-metastatic drug development, and diagnostic applications. Recent advances in structural biology and protein engineering have further refined CDHH designs, improving their functional mimicry of native cadherins while addressing challenges like aggregation and proteolytic sensitivity.
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