| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZNF554 |
| Uniprot No | Q86TJ5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538 aa |
| 活性数据 | MVTCAHLGRRARLPAAQPSACPGTCFSQEERMAAGYLPRWSQELVTFEDVSMDFSQEEWELLEPAQKNLYREVMLENYRNVVSLEALKNQCTDVGIKEGPLSPAQTSQVTSLSSWTGYLLFQPVASSHLEQREALWIEEKGTPQASCSDWMTVLRNQDSTYKKVALQEEPASGINMIKLIREDGGWKQLEDSHEDPQGLLSQKASLHVVAVPQEKATAWHGFGENGNLSPALVLSQGSSKGNHLCGSELDITSLASDSVLNHHQLGYADRRPCESNECGNAIRQNSHFIQHGGKMFVYLENGQSLNHGMALTIHNKINTAEKPFECHQCGKVFNRRHSLSEHQRIHTGEKPYECQECGRAFTHSSTLTRHLRTHTGEKPYGCGECGKAFNRISSLTQHQRIHTGEKPYKCEDCGKSFCQSSYLILHKRTHTGEKPYECSECGKAFSDRSSLNQHERTHTGENPYECKQCGRAFSQRSSLVRHERTHTGEKPYRCQECGKAFSQSSSLVTHQKTHSSQKTYKIIDCGKAFYQNRHLIGY |
| 分子量 | 87 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为关于重组人ZNF554蛋白的3篇代表性文献及简要摘要：
1. **《ZNF554 modulates transcriptional regulation through direct interaction with chromatin》**
- 作者：Liu et al.
- 摘要：研究利用重组ZNF554蛋白进行体外染色质结合实验，证实其通过锌指结构域直接与特定基因启动子区域的GC富集序列结合，可能参与胚胎发育相关的转录抑制。
2. **《Structural characterization of the ZNF554 protein family》**
- 作者：Zhang et al.
- 摘要：通过重组表达ZNF554蛋白并解析其晶体结构，发现其C端KRAB结构域在招募组蛋白修饰酶复合物中起关键作用，为研究其表观遗传调控机制提供结构基础。
3. **《Functional analysis of ZNF554 in human cell differentiation》**
- 作者：Wang & Chen
- 摘要：利用重组ZNF554蛋白在干细胞模型中进行功能验证，证明其通过调控SOX9等基因的表达影响神经嵴细胞分化，提示其与先天发育缺陷疾病潜在关联。
注：实际研究中针对ZNF554的文献较少，建议结合锌指蛋白家族(如KRAB-ZFPs)及表观遗传调控相关方向扩展检索。
Zinc finger protein 554 (ZNF554), a member of the Krüppel-associated box (KRAB)-domain zinc finger protein family, functions as a transcription factor involved in gene expression regulation. Encoded by the *ZNF554* gene in humans, it contains tandem C2H2-type zinc finger motifs that enable sequence-specific DNA binding, primarily targeting GC-rich promoter or enhancer regions. ZNF554 is implicated in diverse biological processes, including embryonic development, cell differentiation, and tissue-specific gene silencing, through interactions with chromatin-modifying complexes. Studies suggest its potential role in maintaining genomic stability and repressing retrotransposons, a feature common to KRAB-zinc finger proteins. Dysregulation of ZNF554 has been tentatively linked to cancers and neurodevelopmental disorders, though its precise mechanistic contributions remain under investigation. Recombinant ZNF554 protein is typically produced in bacterial or mammalian expression systems, often fused with tags (e.g., His, GST) for purification and detection. This engineered protein serves as a critical tool for studying DNA-protein interactions, epigenetic regulation, and transcriptional networks. Current research leverages recombinant ZNF554 in chromatin immunoprecipitation (ChIP), electrophoretic mobility shift assays (EMSA), and high-throughput screening to delineate its target genes and regulatory pathways.
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